Influence of Cytokinins in Combination with GA3 on Shoot Multiplication and Elongation of Tea Clone Iran 100 (Camellia sinensis (L.) O. Kuntze)
Joint Authors
Sinniah, Uma Rani
Gonbad, Reza Azadi
Abdul Aziz, Maheran
Mohamad, Rosfarizan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-28
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The use of in vitro culture has been accepted as an efficient technique for clonal propagation of many woody plants.
In the present research, we report the results of a number of experiments aimed at optimizing micropropagation protocol for tea (Camellia sinensis (L.) O.
Kuntze) (clone Iran 100) using nodal segments as the explant.
The effect of different combinations and concentrations of plant growth regulators (PGR) (BAP, TDZ, GA3) on shoot multiplication and elongation was assessed.
The influence of exposure to IBA in liquid form prior to transfer to solid media on rooting of tea microshoots was investigated.
The results of this study showed that the best treatment for nodal segment multiplication in terms of the number of shoot per explant and shoot elongation was obtained using 3 mg/L BAP in combination with 0.5 mg/L GA3.
TDZ was found to be inappropriate for multiplication of tea clone Iran 100 as it resulted in hyperhydricity especially at concentrations higher than 0.05 mg/L.
Healthy shoots treated with 300 mg/L IBA for 30 min followed by transfer to 1/2 strength MS medium devoid of PGR resulted in 72.3% of shoots producing roots and upon transferring them to acclimatization chamber 65% survival was obtained prior to field transfer.
American Psychological Association (APA)
Gonbad, Reza Azadi& Sinniah, Uma Rani& Abdul Aziz, Maheran& Mohamad, Rosfarizan. 2014. Influence of Cytokinins in Combination with GA3 on Shoot Multiplication and Elongation of Tea Clone Iran 100 (Camellia sinensis (L.) O. Kuntze). The Scientific World Journal،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1051691
Modern Language Association (MLA)
Gonbad, Reza Azadi…[et al.]. Influence of Cytokinins in Combination with GA3 on Shoot Multiplication and Elongation of Tea Clone Iran 100 (Camellia sinensis (L.) O. Kuntze). The Scientific World Journal No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1051691
American Medical Association (AMA)
Gonbad, Reza Azadi& Sinniah, Uma Rani& Abdul Aziz, Maheran& Mohamad, Rosfarizan. Influence of Cytokinins in Combination with GA3 on Shoot Multiplication and Elongation of Tea Clone Iran 100 (Camellia sinensis (L.) O. Kuntze). The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1051691
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051691