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Molecular Characterization of Enterohemorrhagic E. coli O157 Isolated from Animal Fecal and Food Samples in Eastern China
Joint Authors
Wang, Shaohui
Zhang, Shuxiao
Liu, Zhe
Liu, Pingping
Shi, Zixue
Wei, Jianchao
Shao, Donghua
Li, Beibei
Ma, Zhiyong
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-04
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Objective.
To elucidate the extent of food contamination by enterohemorrhagic Escherichia coli (EHEC) O157 in Eastern China.
Methods.
A total of 1100 food and animal fecal samples were screened for EHEC O157.
Then, molecular characterization of each isolate was determined.
Results.
EHEC O157 was isolated as follows: pig feces, 4% (20/500); cattle feces, 3.3% (2/60); chicken feces, 1.43% (2/140); pork, 2.14% (3/140), milk, 1.67% (1/60); and chicken meat, 1.67% (1/60).
The stx1, stx2, eae, and hlyA genes were present in 26.7% (8/30), 40% (12/30), 63.3% (19/30), and 50% (15/30) of the O157 isolates, respectively.
Molecular typing showed that strains from fecal and food samples were clustered into the same molecular typing group.
Furthermore, the isolates from pork and pig feces possessed the same characterization as the clinical strains ATCC35150 and ATCC43889.
Biofilm formation assays showed that 53.3% of the EHEC O157 isolates could produce biofilm.
However, composite analyses showed that biofilm formation of EHEC O157 was independent of genetic background.
Conclusions.
Animal feces, especially from pigs, serve as reservoirs for food contamination by EHEC O157.
Thus, it is important to control contamination by EHEC O157 on farms and in abattoirs to reduce the incidence of foodborne infections in humans.
American Psychological Association (APA)
Wang, Shaohui& Zhang, Shuxiao& Liu, Zhe& Liu, Pingping& Shi, Zixue& Wei, Jianchao…[et al.]. 2014. Molecular Characterization of Enterohemorrhagic E. coli O157 Isolated from Animal Fecal and Food Samples in Eastern China. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1051701
Modern Language Association (MLA)
Wang, Shaohui…[et al.]. Molecular Characterization of Enterohemorrhagic E. coli O157 Isolated from Animal Fecal and Food Samples in Eastern China. The Scientific World Journal No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1051701
American Medical Association (AMA)
Wang, Shaohui& Zhang, Shuxiao& Liu, Zhe& Liu, Pingping& Shi, Zixue& Wei, Jianchao…[et al.]. Molecular Characterization of Enterohemorrhagic E. coli O157 Isolated from Animal Fecal and Food Samples in Eastern China. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1051701
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051701