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Efficient Reprogramming of Mouse Fibroblasts to Neuronal Cells including Dopaminergic Neurons
Joint Authors
Park, Hang-soo
Hwang, Insik
Park, Han-kyul
Choi, Kyung-Ah
Jeong, Hyesun
Kim, Suhng Wook
Oh, Seung-Ick
Hong, Sunghoi
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Somatic cells were directly converted to functional neurons through the use of a combination of transcription factors, including Ascl1, Brn2, and Myt1l.
However, a major limitation is the lack of a reliable source of cell-replacement therapy for neurological diseases.
Here, we show that a combination of the transcription factors Ascl1 and Nurr1 (AN) and neurotrophic factors including SHH and FGF8b directly reprogrammed embryonic mouse fibroblasts to induced neuronal (iN) cells: pan-neuronal cells and dopaminergic (DA) neurons under our systematic cell culture conditions.
Reprogrammed cells showed the morphological properties of neuronal cells.
Additionally, cells were analyzed using various markers, including Tuj1 and Map2 for neuronal cells and Lmx1a, Th, Aadc and Vmat2 for DA neurons in our immunostaining and reverse transcription (RT)-PCR experiments.
We found that a combination of transcription factors and neurotrophic factors could directly reprogram fibroblasts to neuronal cells including DA neurons.
Various types of reprogrammed cells are promising cell sources for cell-based therapy of neurological disorders like Parkinson’s disease and spinal cord injury.
American Psychological Association (APA)
Oh, Seung-Ick& Park, Hang-soo& Hwang, Insik& Park, Han-kyul& Choi, Kyung-Ah& Jeong, Hyesun…[et al.]. 2014. Efficient Reprogramming of Mouse Fibroblasts to Neuronal Cells including Dopaminergic Neurons. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1051749
Modern Language Association (MLA)
Oh, Seung-Ick…[et al.]. Efficient Reprogramming of Mouse Fibroblasts to Neuronal Cells including Dopaminergic Neurons. The Scientific World Journal No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1051749
American Medical Association (AMA)
Oh, Seung-Ick& Park, Hang-soo& Hwang, Insik& Park, Han-kyul& Choi, Kyung-Ah& Jeong, Hyesun…[et al.]. Efficient Reprogramming of Mouse Fibroblasts to Neuronal Cells including Dopaminergic Neurons. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1051749
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051749