Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line
Joint Authors
Conde, Sandro José
Carraro, Dirce Maria
Figueiredo, Nancy Bueno
Cestari, Sílvia Helena
Perone, Denise
Katayama, Maria Lúcia Hirata
Brentani, Helena Paula
Brentani, Maria Mitzi
Síbio, Maria Teresa de
Nogueira, Célia Regina
Luvizotto, Renata de Azevedo Melo
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-23
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
It has been well established that estrogen plays an important role in the progression and treatment of breast cancer.
However, the role of triiodothyronine (T3) remains controversial.
We have previously shown its capacity to stimulate the development of positive estrogen receptor breast carcinoma, induce the expression of genes (PR, TGF-alpha) normally stimulated by estradiol (E2), and suppress genes (TGF-beta) normally inhibited by E2.
Since T3 regulates growth hormones, metabolism, and differentiation, it is important to verify its action on other genes normally induced by E2.
Therefore, we used DNA microarrays to compare gene expression patterns in MCF-7 breast adenocarcinoma cells treated with E2 and T3.
Several genes were modulated by both E2 and T3 in MCF-7 cells (Student’s t-test, P<0.05).
Specifically, we found eight genes that were differentially expressed after treatment with both E2 and T3, including amphiregulin, fibulin 1, claudin 6, pericentriolar material 1, premature ovarian failure 1B, factor for adipocyte differentiation-104, sterile alpha motif domain containing 9, and likely ortholog of rat vacuole membrane protein 1 (fold change > 2.0, pFDR < 0.05).
We confirmed our microarray results by real-time PCR.
Our findings reveal that certain genes in MCF-7 cells can be regulated by both E2 and T3.
American Psychological Association (APA)
Figueiredo, Nancy Bueno& Cestari, Sílvia Helena& Conde, Sandro José& Luvizotto, Renata de Azevedo Melo& Síbio, Maria Teresa de& Perone, Denise…[et al.]. 2014. Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1051804
Modern Language Association (MLA)
Figueiredo, Nancy Bueno…[et al.]. Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line. The Scientific World Journal No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1051804
American Medical Association (AMA)
Figueiredo, Nancy Bueno& Cestari, Sílvia Helena& Conde, Sandro José& Luvizotto, Renata de Azevedo Melo& Síbio, Maria Teresa de& Perone, Denise…[et al.]. Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1051804
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051804