Validation of a Real Time PCR for Classical Swine Fever Diagnosis
Joint Authors
Sales, Érica Bravo
Dias, Natanael Lamas
Fonseca Júnior, Antônio Augusto
Oliveira, Anapolino Macedo
Alves, Bruna Rios Coelho
Dorella, Fernanda Alves
Camargos, Marcelo Fernandes
Source
Veterinary Medicine International
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-09
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
The viral disease classical swine fever (CSF), caused by a Pestivirus, is one of the major causes of economic losses for pig farming.
The aim of this work was to validate a RT-qPCR using Taqman for detection of CSF in swine tissues.
The parameters for the validation followed the specifications of the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals of the World Organization for Animal Health (OIE) and the guide ABNT NBR ISO/IEC 17025:2005.
The analysis of the 5′NTR region of CSF virus was performed in 145 samples from 29 infected pigs and in 240 samples from 80 pigs originated in the Brazilian CSF-free zone.
The tissues tested were spleen, kidney, blood, tonsils, and lymph nodes.
Sequencing of the positive samples for 5′NTR region was performed to evaluate the specificity of the RT-qPCR.
Tests performed for the RT-qPCR validation demonstrated that the PCR assay was efficient in detecting RNA from CSF virus in all materials from different tissues of infected animals.
Furthermore, RNA from CSF virus was not detected in samples of swine originated from the Brazilian CSF-free zone.
Hence, it is concluded that RT-qPCR can be used as a complementary diagnostic for CSF.
American Psychological Association (APA)
Dias, Natanael Lamas& Fonseca Júnior, Antônio Augusto& Oliveira, Anapolino Macedo& Sales, Érica Bravo& Alves, Bruna Rios Coelho& Dorella, Fernanda Alves…[et al.]. 2014. Validation of a Real Time PCR for Classical Swine Fever Diagnosis. Veterinary Medicine International،Vol. 2014, no. 2014, pp.1-4.
https://search.emarefa.net/detail/BIM-1051892
Modern Language Association (MLA)
Dias, Natanael Lamas…[et al.]. Validation of a Real Time PCR for Classical Swine Fever Diagnosis. Veterinary Medicine International No. 2014 (2014), pp.1-4.
https://search.emarefa.net/detail/BIM-1051892
American Medical Association (AMA)
Dias, Natanael Lamas& Fonseca Júnior, Antônio Augusto& Oliveira, Anapolino Macedo& Sales, Érica Bravo& Alves, Bruna Rios Coelho& Dorella, Fernanda Alves…[et al.]. Validation of a Real Time PCR for Classical Swine Fever Diagnosis. Veterinary Medicine International. 2014. Vol. 2014, no. 2014, pp.1-4.
https://search.emarefa.net/detail/BIM-1051892
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051892