Establishment, Culture, and Characterization of Guinea Pig Fetal Fibroblast Cell
Joint Authors
Mahboobi, Reza
Dianatpour, Mehdi
Zare, Shahrokh
Tamadon, Amin
Hosseini, Seyed Ebrahim
Mehrabani, Davood
Source
Veterinary Medicine International
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-25
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Establishment of Guinea pig fetal fibroblast cells and their biological evaluation before and after cryopreservation were the main purposes of this study.
After determination of the proper age of pregnancy by ultrasonography, 30 days old fetuses of Guinea pigs were recovered.
Their skins were cut into small pieces (1 mm2) and were cultured.
When reaching 80–90% confluence, the cells were passaged.
Cells of the second and eighth passages were cultured in 24-well plates ( 4 × 10 4 cells/well) for 6 days and three wells per day were counted.
The average cell counts at each time point were then plotted against time and the population doubling time (PDT) was determined.
Then, vials of cells ( 2 × 10 6 cells/mL) were cryopreserved for 1 month and after thawing, the cell viability was evaluated.
The PDT of the second passage was about 23 h and for the eighth passage was about 30 h.
The viability of the cultures was 95% in the second passage and 74.5% in the eighth passage.
It was shown that the Guinea pig fetal fibroblast cell culture can be established using the adherent culture method while, after freezing, the viability indices of these cells were favorable.
American Psychological Association (APA)
Mehrabani, Davood& Mahboobi, Reza& Dianatpour, Mehdi& Zare, Shahrokh& Tamadon, Amin& Hosseini, Seyed Ebrahim. 2014. Establishment, Culture, and Characterization of Guinea Pig Fetal Fibroblast Cell. Veterinary Medicine International،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1051927
Modern Language Association (MLA)
Mehrabani, Davood…[et al.]. Establishment, Culture, and Characterization of Guinea Pig Fetal Fibroblast Cell. Veterinary Medicine International No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-1051927
American Medical Association (AMA)
Mehrabani, Davood& Mahboobi, Reza& Dianatpour, Mehdi& Zare, Shahrokh& Tamadon, Amin& Hosseini, Seyed Ebrahim. Establishment, Culture, and Characterization of Guinea Pig Fetal Fibroblast Cell. Veterinary Medicine International. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1051927
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051927