Electrophoretic Analysis of Indian Isolates of Mycoplasma agalactiae and Mycoplasma bovis by SDS-PAGE and Immunoblotting
Joint Authors
Kumar, Amit
Singh, Vijendra Pal
Srivastava, N. C.
Sunder, Jai
Source
Veterinary Medicine International
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-03
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Mycoplasma agalactiae and Mycoplasma bovis both are responsible for respiratory conditions in sheep and goats.
M.
agalactiae is a major pathogen of sheep and goats and accounts for almost 90% of outbreaks of contagious agalactia syndrome in goats and almost 100% in sheep.
On the basis of clinical signs and cultural, morphological, and biochemical characterization it is almost impossible to differentiate between both the species.
Moreover, due to presence of genomic and proteomic similarity most of the time routine diagnostic tests fail to differentiate between them.
Hence the present study was conducted to find out the protein profile of isolates of both the species by SDS-PAGE and to find out the cross-reacting as well as differentiating immunogenic proteins by Immunoblotting, which can be of immunoprophylactic as well as diagnostic values.
The study revealed 6-7 major immunogenic cross-reactive proteins with the presence of two important non-cross-reacting species specific polypeptides particularly 25.50 and 24.54 kDa in M.
agalactiae and M.
bovis, respectively, that might be of diagnostic values.
American Psychological Association (APA)
Kumar, Amit& Srivastava, N. C.& Singh, Vijendra Pal& Sunder, Jai. 2014. Electrophoretic Analysis of Indian Isolates of Mycoplasma agalactiae and Mycoplasma bovis by SDS-PAGE and Immunoblotting. Veterinary Medicine International،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1051950
Modern Language Association (MLA)
Kumar, Amit…[et al.]. Electrophoretic Analysis of Indian Isolates of Mycoplasma agalactiae and Mycoplasma bovis by SDS-PAGE and Immunoblotting. Veterinary Medicine International No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1051950
American Medical Association (AMA)
Kumar, Amit& Srivastava, N. C.& Singh, Vijendra Pal& Sunder, Jai. Electrophoretic Analysis of Indian Isolates of Mycoplasma agalactiae and Mycoplasma bovis by SDS-PAGE and Immunoblotting. Veterinary Medicine International. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1051950
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051950