On Data Space Selection and Data Processing for Parameter Identification in a Reaction-Diffusion Model Based on FRAP Experiments
Joint Authors
Kindermann, Stefan
Papáček, Štěpán
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-05
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Fluorescence recovery after photobleaching (FRAP) is a widely used measurement technique to determine the mobility of fluorescent molecules within living cells.
While the experimental setup and protocol for FRAP experiments are usually fixed, data (pre)processing represents an important issue.
The aim of this paper is twofold.
First, we formulate and solve the problem of relevant FRAP data selection.
The theoretical findings are illustrated by the comparison of the results of parameter identification when the full data set was used and the case when the irrelevant data set (data with negligible impact on the confidence interval of the estimated parameters) was removed from the data space.
Second, we analyze and compare two approaches of FRAP data processing.
Our proposition, surprisingly for the FRAP community, claims that the data set represented by the FRAP recovery curves in form of a time series (integrated data approach commonly used by the FRAP community) leads to a larger confidence interval compared to the full (spatiotemporal) data approach.
American Psychological Association (APA)
Kindermann, Stefan& Papáček, Štěpán. 2015. On Data Space Selection and Data Processing for Parameter Identification in a Reaction-Diffusion Model Based on FRAP Experiments. Abstract and Applied Analysis،Vol. 2015, no. 2015, pp.1-17.
https://search.emarefa.net/detail/BIM-1052132
Modern Language Association (MLA)
Kindermann, Stefan& Papáček, Štěpán. On Data Space Selection and Data Processing for Parameter Identification in a Reaction-Diffusion Model Based on FRAP Experiments. Abstract and Applied Analysis No. 2015 (2015), pp.1-17.
https://search.emarefa.net/detail/BIM-1052132
American Medical Association (AMA)
Kindermann, Stefan& Papáček, Štěpán. On Data Space Selection and Data Processing for Parameter Identification in a Reaction-Diffusion Model Based on FRAP Experiments. Abstract and Applied Analysis. 2015. Vol. 2015, no. 2015, pp.1-17.
https://search.emarefa.net/detail/BIM-1052132
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1052132