Effect of Lidocaine and Epinephrine on Human Erythrocyte Shape and Vesiculability of Blood Cells
Joint Authors
Slokar, Tanja
Lopez-Mariscal, Carlos
Krek, Judita Lea
Štukelj, Roman
Zupanc, Oskar
Kralj-Iglič, Veronika
Source
Advances in Condensed Matter Physics
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The effect of local anesthetic composed of lidocaine and epinephrine on vesiculability of blood cells and erythrocyte shape was studied.
Whole blood and plasma were incubated with lidocaine/epinephrine.
Extracellular vesicles were isolated by centrifugation and washing and counted by flow cytometry.
Lidocaine/epinephrine and each component alone were added to diluted blood.
Shape changes were recorded by micrographs.
An ensemble of captured frames was analyzed for populations of discocytes, echinocytes, and stomatocytes by using statistical methods.
Incubation of whole blood and blood plasma with lidocaine/epinephrine considerably increased concentration of extracellular vesicles in isolates (for an average factor 3.4 in blood and 2.8 in plasma).
Lidocaine/epinephrine caused change of erythrocyte shape from mainly discocytic to mainly stomatocytic (higher than 50%).
Lidocaine alone had even stronger stomatocytic effect (the percent of stomatocytes was higher than 95%) while epinephrine had echinocytic effect (the percent of echinocytes was higher than 80%).
The differences were highly statistically significant p<10-8 with statistical power P=1.
Lidocaine/epinephrine induced regions of highly anisotropically curved regions indicating that lidocaine and epinephrine interact with erythrocyte membrane.
It was concluded that lidocaine/epinephrine interacts with cell membranes and increases vesiculability of blood cells in vitro.
American Psychological Association (APA)
Slokar, Tanja& Lopez-Mariscal, Carlos& Krek, Judita Lea& Štukelj, Roman& Zupanc, Oskar& Kralj-Iglič, Veronika. 2015. Effect of Lidocaine and Epinephrine on Human Erythrocyte Shape and Vesiculability of Blood Cells. Advances in Condensed Matter Physics،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1052325
Modern Language Association (MLA)
Slokar, Tanja…[et al.]. Effect of Lidocaine and Epinephrine on Human Erythrocyte Shape and Vesiculability of Blood Cells. Advances in Condensed Matter Physics No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1052325
American Medical Association (AMA)
Slokar, Tanja& Lopez-Mariscal, Carlos& Krek, Judita Lea& Štukelj, Roman& Zupanc, Oskar& Kralj-Iglič, Veronika. Effect of Lidocaine and Epinephrine on Human Erythrocyte Shape and Vesiculability of Blood Cells. Advances in Condensed Matter Physics. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1052325
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1052325