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Low-Dose Ribavirin Treatments Attenuate Neuroinflammatory Activation of BV-2 Cells by Interfering with Inducible Nitric Oxide Synthase
Joint Authors
Bozic, Iva
Savic, Danijela
Jovanovic, Marija
Bjelobaba, Ivana
Laketa, Danijela
Nedeljkovic, Nadezda
Stojiljkovic, Mirjana
Pekovic, Sanja
Lavrnja, Irena
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Microglia play a key role in defending central nervous system from various internal and external threats.
However, their excessive and/or chronic activation is associated with deleterious effects in a variety of neurodegenerative diseases.
Previously, we have shown that ribavirin when applied in clinically relevant dosage (10 μM) modulates activated microglia in complex fashion inducing both anti- and proinflammatory effects, simultaneously causing cytotoxicity.
Here, we examined potential of low-dose ribavirin (0.1 and 1 μM) to modulate activated BV-2 microglia.
Morphological and functional activation of BV-2 cells was achieved with lipopolysaccharide (LPS) stimulation.
Our results demonstrated that low-dose ribavirin did not induce cell death, while 10 μM ribavirin promoted LPS induced apoptosis.
We determined that 1 μM ribavirin was equally efficient in deactivation of LPS induced morphological changes as 10 μM ribavirin treatment.
Ribavirin showed halfway success in reducing markers of functional activation of microglia.
Namely, none of the doses had effect on LPS triggered production of proinflammatory cytokine tumor necrosis factor alpha.
On the other hand, low-dose ribavirin proved its effectiveness in reduction of another inflammatory mediator, nitric oxide, by inhibiting inducible form of nitric oxide synthase.
Our results imply that low-dose ribavirin may alleviate nitrosative stress during neuroinflammation.
American Psychological Association (APA)
Bozic, Iva& Savic, Danijela& Jovanovic, Marija& Bjelobaba, Ivana& Laketa, Danijela& Nedeljkovic, Nadezda…[et al.]. 2015. Low-Dose Ribavirin Treatments Attenuate Neuroinflammatory Activation of BV-2 Cells by Interfering with Inducible Nitric Oxide Synthase. Analytical Cellular Pathology،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1052375
Modern Language Association (MLA)
Bozic, Iva…[et al.]. Low-Dose Ribavirin Treatments Attenuate Neuroinflammatory Activation of BV-2 Cells by Interfering with Inducible Nitric Oxide Synthase. Analytical Cellular Pathology No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1052375
American Medical Association (AMA)
Bozic, Iva& Savic, Danijela& Jovanovic, Marija& Bjelobaba, Ivana& Laketa, Danijela& Nedeljkovic, Nadezda…[et al.]. Low-Dose Ribavirin Treatments Attenuate Neuroinflammatory Activation of BV-2 Cells by Interfering with Inducible Nitric Oxide Synthase. Analytical Cellular Pathology. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1052375
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1052375