Archaeal Nucleic Acid Ligases and Their Potential in Biotechnology
Joint Authors
Chambers, Cecilia R.
Patrick, Wayne M.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-01
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
With their ability to catalyse the formation of phosphodiester linkages, DNA ligases and RNA ligases are essential tools for many protocols in molecular biology and biotechnology.
Currently, the nucleic acid ligases from bacteriophage T4 are used extensively in these protocols.
In this review, we argue that the nucleic acid ligases from Archaea represent a largely untapped pool of enzymes with diverse and potentially favourable properties for new and emerging biotechnological applications.
We summarise the current state of knowledge on archaeal DNA and RNA ligases, which makes apparent the relative scarcity of information on in vitro activities that are of most relevance to biotechnologists (such as the ability to join blunt- or cohesive-ended, double-stranded DNA fragments).
We highlight the existing biotechnological applications of archaeal DNA ligases and RNA ligases.
Finally, we draw attention to recent experiments in which protein engineering was used to modify the activities of the DNA ligase from Pyrococcus furiosus and the RNA ligase from Methanothermobacter thermautotrophicus, thus demonstrating the potential for further work in this area.
American Psychological Association (APA)
Chambers, Cecilia R.& Patrick, Wayne M.. 2015. Archaeal Nucleic Acid Ligases and Their Potential in Biotechnology. Archaea،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1053919
Modern Language Association (MLA)
Chambers, Cecilia R.& Patrick, Wayne M.. Archaeal Nucleic Acid Ligases and Their Potential in Biotechnology. Archaea No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1053919
American Medical Association (AMA)
Chambers, Cecilia R.& Patrick, Wayne M.. Archaeal Nucleic Acid Ligases and Their Potential in Biotechnology. Archaea. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1053919
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1053919