From Structure-Function Analyses to Protein Engineering for Practical Applications of DNA Ligase
Joint Authors
Ishino, Yoshizumi
Tanabe, Maiko
Nishida, Hirokazu
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-20, 20 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-05
Country of Publication
Egypt
No. of Pages
20
Main Subjects
Abstract EN
DNA ligases are indispensable in all living cells and ubiquitous in all organs.
DNA ligases are broadly utilized in molecular biology research fields, such as genetic engineering and DNA sequencing technologies.
Here we review the utilization of DNA ligases in a variety of in vitro gene manipulations, developed over the past several decades.
During this period, fewer protein engineering attempts for DNA ligases have been made, as compared to those for DNA polymerases.
We summarize the recent progress in the elucidation of the DNA ligation mechanisms obtained from the tertiary structures solved thus far, in each step of the ligation reaction scheme.
We also present some examples of engineered DNA ligases, developed from the viewpoint of their three-dimensional structures.
American Psychological Association (APA)
Tanabe, Maiko& Ishino, Yoshizumi& Nishida, Hirokazu. 2015. From Structure-Function Analyses to Protein Engineering for Practical Applications of DNA Ligase. Archaea،Vol. 2015, no. 2015, pp.1-20.
https://search.emarefa.net/detail/BIM-1053923
Modern Language Association (MLA)
Tanabe, Maiko…[et al.]. From Structure-Function Analyses to Protein Engineering for Practical Applications of DNA Ligase. Archaea No. 2015 (2015), pp.1-20.
https://search.emarefa.net/detail/BIM-1053923
American Medical Association (AMA)
Tanabe, Maiko& Ishino, Yoshizumi& Nishida, Hirokazu. From Structure-Function Analyses to Protein Engineering for Practical Applications of DNA Ligase. Archaea. 2015. Vol. 2015, no. 2015, pp.1-20.
https://search.emarefa.net/detail/BIM-1053923
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1053923