Recombinant Cyclodextrinase from Thermococcus kodakarensis KOD1: Expression, Purification, and Enzymatic Characterization
Joint Authors
Sun, Ying
Lv, Xiaomin
Li, Zhengqun
Wang, Jiaqiang
Jia, Baolei
Liu, Jinliang
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A gene encoding a cyclodextrinase from Thermococcus kodakarensis KOD1 (CDase-Tk) was identified and characterized.
The gene encodes a protein of 656 amino acid residues with a molecular mass of 76.4 kDa harboring four conserved regions found in all members of the α-amylase family.
A recombinant form of the enzyme was purified by ion-exchange chromatography, and its catalytic properties were examined.
The enzyme was active in a broad range of pH conditions (pHs 4.0–10.0), with an optimal pH of 7.5 and a temperature optimum of 65°C.
The purified enzyme preferred to hydrolyze β-cyclodextrin (CD) but not α- or γ-CD, soluble starch, or pullulan.
The final product from β-CD was glucose.
The Vmax and Km values were 3.13 ± 0.47 U mg−1 and 2.94 ± 0.16 mg mL−1 for β-CD.
The unique characteristics of CDase-Tk with a low catalytic temperature and substrate specificity are discussed, and the starch utilization pathway in a broad range of temperatures is also proposed.
American Psychological Association (APA)
Sun, Ying& Lv, Xiaomin& Li, Zhengqun& Wang, Jiaqiang& Jia, Baolei& Liu, Jinliang. 2015. Recombinant Cyclodextrinase from Thermococcus kodakarensis KOD1: Expression, Purification, and Enzymatic Characterization. Archaea،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1053926
Modern Language Association (MLA)
Sun, Ying…[et al.]. Recombinant Cyclodextrinase from Thermococcus kodakarensis KOD1: Expression, Purification, and Enzymatic Characterization. Archaea No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1053926
American Medical Association (AMA)
Sun, Ying& Lv, Xiaomin& Li, Zhengqun& Wang, Jiaqiang& Jia, Baolei& Liu, Jinliang. Recombinant Cyclodextrinase from Thermococcus kodakarensis KOD1: Expression, Purification, and Enzymatic Characterization. Archaea. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1053926
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1053926