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Molecular Analysis of Methanogen Richness in Landfill and Marshland Targeting 16S rDNA Sequences
Joint Authors
Yadav, Shailendra
Kundu, Sharbadeb
Ghosh, Sankar K.
Maitra, S. S.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Methanogens, a key contributor in global carbon cycling, methane emission, and alternative energy production, generate methane gas via anaerobic digestion of organic matter.
The methane emission potential depends upon methanogenic diversity and activity.
Since they are anaerobes and difficult to isolate and culture, their diversity present in the landfill sites of Delhi and marshlands of Southern Assam, India, was analyzed using molecular techniques like 16S rDNA sequencing, DGGE, and qPCR.
The sequencing results indicated the presence of methanogens belonging to the seventh order and also the order Methanomicrobiales in the Ghazipur and Bhalsawa landfill sites of Delhi.
Sequences, related to the phyla Crenarchaeota (thermophilic) and Thaumarchaeota (mesophilic), were detected from marshland sites of Southern Assam, India.
Jaccard analysis of DGGE gel using Gel2K showed three main clusters depending on the number and similarity of band patterns.
The copy number analysis of hydrogenotrophic methanogens using qPCR indicates higher abundance in landfill sites of Delhi as compared to the marshlands of Southern Assam.
The knowledge about “methanogenic archaea composition” and “abundance” in the contrasting ecosystems like “landfill” and “marshland” may reorient our understanding of the Archaea inhabitants.
This study could shed light on the relationship between methane-dynamics and the global warming process.
American Psychological Association (APA)
Yadav, Shailendra& Kundu, Sharbadeb& Ghosh, Sankar K.& Maitra, S. S.. 2015. Molecular Analysis of Methanogen Richness in Landfill and Marshland Targeting 16S rDNA Sequences. Archaea،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1053929
Modern Language Association (MLA)
Yadav, Shailendra…[et al.]. Molecular Analysis of Methanogen Richness in Landfill and Marshland Targeting 16S rDNA Sequences. Archaea No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1053929
American Medical Association (AMA)
Yadav, Shailendra& Kundu, Sharbadeb& Ghosh, Sankar K.& Maitra, S. S.. Molecular Analysis of Methanogen Richness in Landfill and Marshland Targeting 16S rDNA Sequences. Archaea. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1053929
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1053929