A Novel Highly Thermostable Multifunctional Beta-Glycosidase from Crenarchaeon Acidilobus saccharovorans
Joint Authors
Gumerov, Vadim M.
Rakitin, Andrey L.
Mardanov, Andrey V.
Ravin, Nikolai V.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-11
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
We expressed a putative β-galactosidase Asac_1390 from hyperthermophilic crenarchaeon Acidilobus saccharovorans in Escherichia coli and purified the recombinant enzyme.
Asac_1390 is composed of 490 amino acid residues and showed high sequence similarity to family 1 glycoside hydrolases from various thermophilic Crenarchaeota.
The maximum activity was observed at pH 6.0 and 93°C.
The half-life of the enzyme at 90°C was about 7 hours.
Asac_1390 displayed high tolerance to glucose and exhibits hydrolytic activity towards cellobiose and various aryl glucosides.
The hydrolytic activity with p-nitrophenyl (pNP) substrates followed the order pNP-β-D-galactopyranoside (328 U mg−1), pNP-β-D-glucopyranoside (246 U mg−1), pNP-β-D-xylopyranoside (72 U mg−1), and pNP-β-D-mannopyranoside (28 U mg−1).
Thus the enzyme was actually a multifunctional β-glycosidase.
Therefore, the utilization of Asac_1390 may contribute to facilitating the efficient degradation of lignocellulosic biomass and help enhance bioconversion processes.
American Psychological Association (APA)
Gumerov, Vadim M.& Rakitin, Andrey L.& Mardanov, Andrey V.& Ravin, Nikolai V.. 2015. A Novel Highly Thermostable Multifunctional Beta-Glycosidase from Crenarchaeon Acidilobus saccharovorans. Archaea،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1053939
Modern Language Association (MLA)
Gumerov, Vadim M.…[et al.]. A Novel Highly Thermostable Multifunctional Beta-Glycosidase from Crenarchaeon Acidilobus saccharovorans. Archaea No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1053939
American Medical Association (AMA)
Gumerov, Vadim M.& Rakitin, Andrey L.& Mardanov, Andrey V.& Ravin, Nikolai V.. A Novel Highly Thermostable Multifunctional Beta-Glycosidase from Crenarchaeon Acidilobus saccharovorans. Archaea. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1053939
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1053939