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Expression of Factor X in BHK-21 Cells Promotes Low Pathogenic Influenza Viruses Replication
Joint Authors
Shahsavandi, Shahla
Ebrahimi, Mohammad Majid
Masoudi, Shahin
Izadi, Hasan
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-31
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
A cDNA clone for factor 10 (FX) isolated from chicken embryo inserted into the mammalian cell expression vector pCDNA3.1 was transfected into the baby hamster kidney (BHK-21) cell line.
The generated BHK-21 cells with inducible expression of FX were used to investigate the efficacy of the serine transmembrane protease to proteolytic activation of influenza virus hemagglutinin (HA) with monobasic cleavage site.
Data showed that the BHK-21/FX stably expressed FX after ten serial passages.
The cells could proteolytically cleave the HA of low pathogenic avian influenza virus at multiplicity of infection 0.01.
Growth kinetics of the virus on BHK-21/FX, BHK-21, and MDCK cells were evaluated by titrations of virus particles in each culture supernatant.
Efficient multicycle viral replication was markedly detected in the cell at subsequent passages.
Virus titration demonstrated that BHK-21/FX cell supported high-titer growth of the virus in which the viral titer is comparable to the virus grown in BHK-21 or MDCK cells with TPCK-trypsin.
The results indicate potential application for the BHK-21/FX in influenza virus replication procedure and related studies.
American Psychological Association (APA)
Shahsavandi, Shahla& Ebrahimi, Mohammad Majid& Masoudi, Shahin& Izadi, Hasan. 2015. Expression of Factor X in BHK-21 Cells Promotes Low Pathogenic Influenza Viruses Replication. Advances in Virology،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1054030
Modern Language Association (MLA)
Shahsavandi, Shahla…[et al.]. Expression of Factor X in BHK-21 Cells Promotes Low Pathogenic Influenza Viruses Replication. Advances in Virology No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1054030
American Medical Association (AMA)
Shahsavandi, Shahla& Ebrahimi, Mohammad Majid& Masoudi, Shahin& Izadi, Hasan. Expression of Factor X in BHK-21 Cells Promotes Low Pathogenic Influenza Viruses Replication. Advances in Virology. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1054030
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054030