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Nelfinavir Impairs Glycosylation of Herpes Simplex Virus 1 Envelope Proteins and Blocks Virus Maturation
Joint Authors
Gantt, Soren
Gachelet, Eliora
Carlsson, Jacquelyn
Barcy, Serge
Lagunoff, Michael
Casper, Corey
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Nelfinavir (NFV) is an HIV-1 aspartyl protease inhibitor that has numerous effects on human cells, which impart attractive antitumor properties.
NFV has also been shown to have in vitro inhibitory activity against human herpesviruses (HHVs).
Given the apparent absence of an aspartyl protease encoded by HHVs, we investigated the mechanism of action of NFV herpes simplex virus type 1 (HSV-1) in cultured cells.
Selection of HSV-1 resistance to NFV was not achieved despite multiple passages under drug pressure.
NFV did not significantly affect the level of expression of late HSV-1 gene products.
Normal numbers of viral particles appeared to be produced in NFV-treated cells by electron microscopy but remain within the cytoplasm more often than controls.
NFV did not inhibit the activity of the HSV-1 serine protease nor could its antiviral activity be attributed to inhibition of Akt phosphorylation.
NFV was found to decrease glycosylation of viral glycoproteins B and C and resulted in aberrant subcellular localization, consistent with induction of endoplasmic reticulum stress and the unfolded protein response by NFV.
These results demonstrate that NFV causes alterations in HSV-1 glycoprotein maturation and egress and likely acts on one or more host cell functions that are important for HHV replication.
American Psychological Association (APA)
Gantt, Soren& Gachelet, Eliora& Carlsson, Jacquelyn& Barcy, Serge& Casper, Corey& Lagunoff, Michael. 2015. Nelfinavir Impairs Glycosylation of Herpes Simplex Virus 1 Envelope Proteins and Blocks Virus Maturation. Advances in Virology،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1054031
Modern Language Association (MLA)
Gantt, Soren…[et al.]. Nelfinavir Impairs Glycosylation of Herpes Simplex Virus 1 Envelope Proteins and Blocks Virus Maturation. Advances in Virology No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1054031
American Medical Association (AMA)
Gantt, Soren& Gachelet, Eliora& Carlsson, Jacquelyn& Barcy, Serge& Casper, Corey& Lagunoff, Michael. Nelfinavir Impairs Glycosylation of Herpes Simplex Virus 1 Envelope Proteins and Blocks Virus Maturation. Advances in Virology. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1054031
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054031