Recognition Code of ZNF191(243-368)‎ and Its Interaction with DNA

Abstract EN

ZNF191(243-368) is the C-terminal region of ZNF191 which contains a putative DNA-binding domain of four Cys2His2 zinc finger motifs.

In this study, an expression vector of a fusion protein of ZNF191(243-368) with glutathione-S-transferase (GST) was constructed and transformed into Escherichia coli BL21.

The fusion protein GST-ZNF191(243-368) was expressed using this vector to investigate the protein-DNA binding reaction through an affinity selection strategy on the basis of the binding quality of the zinc finger domain.

Results showed that ZNF191(243-368) can selectively bind with sequences and react with genes which contain an AGGG core.

However, the recognition mechanism of Cys2His2 zinc finger proteins to DNA warrants further investigation.