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Early-Onset Diabetic E1-DN Mice Develop Albuminuria and Glomerular Injury Typical of Diabetic Nephropathy
Joint Authors
Hyvönen, Mervi E.
Dumont, Vincent
Tienari, Jukka
Lehtonen, Eero
Ustinov, Jarkko
Havana, Marika
Jalanko, Hannu
Otonkoski, Timo
Miettinen, Päivi J.
Lehtonen, Sanna
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-27
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The transgenic E1-DN mice express a kinase-negative epidermal growth factor receptor in their pancreatic islets and are diabetic from two weeks of age due to impaired postnatal growth of β-cell mass.
Here, we characterize the development of hyperglycaemia-induced renal injury in the E1-DN mice.
Homozygous mice showed increased albumin excretion rate (AER) at the age of 10 weeks; the albuminuria increased over time and correlated with blood glucose.
Morphometric analysis of PAS-stained histological sections and electron microscopy images revealed mesangial expansion in homozygous E1-DN mice, and glomerular sclerosis was observed in the most hyperglycaemic mice.
The albuminuric homozygous mice developed also other structural changes in the glomeruli, including thickening of the glomerular basement membrane and widening of podocyte foot processes that are typical for diabetic nephropathy.
Increased apoptosis of podocytes was identified as one mechanism contributing to glomerular injury.
In addition, nephrin expression was reduced in the podocytes of albuminuric homozygous E1-DN mice.
Tubular changes included altered epithelial cell morphology and increased proliferation.
In conclusion, hyperglycaemic E1-DN mice develop albuminuria and glomerular and tubular injury typical of human diabetic nephropathy and can serve as a new model to study the mechanisms leading to the development of diabetic nephropathy.
American Psychological Association (APA)
Hyvönen, Mervi E.& Dumont, Vincent& Tienari, Jukka& Lehtonen, Eero& Ustinov, Jarkko& Havana, Marika…[et al.]. 2015. Early-Onset Diabetic E1-DN Mice Develop Albuminuria and Glomerular Injury Typical of Diabetic Nephropathy. BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1054212
Modern Language Association (MLA)
Hyvönen, Mervi E.…[et al.]. Early-Onset Diabetic E1-DN Mice Develop Albuminuria and Glomerular Injury Typical of Diabetic Nephropathy. BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1054212
American Medical Association (AMA)
Hyvönen, Mervi E.& Dumont, Vincent& Tienari, Jukka& Lehtonen, Eero& Ustinov, Jarkko& Havana, Marika…[et al.]. Early-Onset Diabetic E1-DN Mice Develop Albuminuria and Glomerular Injury Typical of Diabetic Nephropathy. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1054212
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054212