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Development of Enhanced Primer Sets for Detection of Norovirus
Joint Authors
Lee, Sung-Geun
Jheong, Weon-Hwa
Paik, Soon-Young
Kong, Byoung-Hwa
Han, Sang-Ha
Jin, Ji-Young
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-28
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Norovirus (NV) is a major viral pathogen that causes nonbacterial acute gastroenteritis and outbreaks of food-borne disease.
The genotype of NV most frequently responsible for NV outbreaks is GII.4, which accounts for 60–80% of cases.
Moreover, original and new NV variant types have been continuously emerging, and their emergence is related to the recent global increase in NV infection.
In this study, we developed advanced primer sets (NKI-F/R/F2, NKII-F/R/R2) for the detection of NV, including the variant types.
The new primer sets were compared with conventional primer sets (GI-F1/R1/F2, SRI-1/2/3, GII-F1/R1/F2, and SRII-1/2/3) to evaluate their efficiency when using clinical and environmental samples.
Using reverse transcription polymerase chain reaction (RT-PCR) and seminested PCR, NV GI and GII were detected in 91.7% (NKI-F/R/F2), 89.3% (NKII-F/R/R2), 54.2% (GI-F1/R1/F2), 52.5% (GII-F1/R1/F2), 25.0% (SRI-1/2/3), and 32.2% (SRII-1/2/3) of clinical and environmental specimens.
Therefore, our primer sets perform better than conventional primer sets in the detection of emerged types of NV and could be used in the future for epidemiological diagnosis of infection with the virus.
American Psychological Association (APA)
Kong, Byoung-Hwa& Lee, Sung-Geun& Han, Sang-Ha& Jin, Ji-Young& Jheong, Weon-Hwa& Paik, Soon-Young. 2015. Development of Enhanced Primer Sets for Detection of Norovirus. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1054214
Modern Language Association (MLA)
Kong, Byoung-Hwa…[et al.]. Development of Enhanced Primer Sets for Detection of Norovirus. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1054214
American Medical Association (AMA)
Kong, Byoung-Hwa& Lee, Sung-Geun& Han, Sang-Ha& Jin, Ji-Young& Jheong, Weon-Hwa& Paik, Soon-Young. Development of Enhanced Primer Sets for Detection of Norovirus. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1054214
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054214