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In Vitro Evaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds
Joint Authors
Egaña, José T.
Schenck, Thilo L.
Wahl, Elizabeth A.
Fierro, Fernando A.
Peavy, Thomas R.
Hopfner, Ursula
Dye, Julian F.
Machens, Hans-Günther
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-04
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Mesenchymal stem cells (MSCs) have been shown to improve tissue regeneration in several preclinical and clinical trials.
These cells have been used in combination with three-dimensional scaffolds as a promising approach in the field of regenerative medicine.
We compare the behavior of human adipose-derived MSCs (AdMSCs) on four different biomaterials that are awaiting or have already received FDA approval to determine a suitable regenerative scaffold for delivering these cells to dermal wounds and increasing healing potential.
AdMSCs were isolated, characterized, and seeded onto scaffolds based on chitosan, fibrin, bovine collagen, and decellularized porcine dermis.
In vitro results demonstrated that the scaffolds strongly influence key parameters, such as seeding efficiency, cellular distribution, attachment, survival, metabolic activity, and paracrine release.
Chick chorioallantoic membrane assays revealed that the scaffold composition similarly influences the angiogenic potential of AdMSCs in vivo.
The wound healing potential of scaffolds increases by means of a synergistic relationship between AdMSCs and biomaterial resulting in the release of proangiogenic and cytokine factors, which is currently lacking when a scaffold alone is utilized.
Furthermore, the methods used herein can be utilized to test other scaffold materials to increase their wound healing potential with AdMSCs.
American Psychological Association (APA)
Wahl, Elizabeth A.& Fierro, Fernando A.& Peavy, Thomas R.& Hopfner, Ursula& Dye, Julian F.& Machens, Hans-Günther…[et al.]. 2015. In Vitro Evaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds. BioMed Research International،Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1054243
Modern Language Association (MLA)
Wahl, Elizabeth A.…[et al.]. In Vitro Evaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds. BioMed Research International No. 2015 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1054243
American Medical Association (AMA)
Wahl, Elizabeth A.& Fierro, Fernando A.& Peavy, Thomas R.& Hopfner, Ursula& Dye, Julian F.& Machens, Hans-Günther…[et al.]. In Vitro Evaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1054243
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054243