Comparative Study of the Accuracy of Different Techniques for the Laboratory Diagnosis of Schistosomiasis Mansoni in Areas of Low Endemicity in Barra Mansa City, Rio de Janeiro State, Brazil
Joint Authors
Carrilho, Flair J.
Espírito-Santo, Maria Cristina Carvalho
Alvarado-Mora, Mónica Viviana
Pinto, Pedro Luiz Silva
Sanchez, Maria Carmen Arroyo
Dias-Neto, Emmanuel
Castilho, Vera Lúcia Pagliusi
Gonçalves, Elenice Messias do Nascimento
Chieffi, Pedro Paulo
Luna, Expedito José de Albuquerque
Pinho, João Renato Rebello
Gryschek, Ronaldo Cesar Borges
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-04
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Schistosomiasis constitutes a major public health problem, with an estimated 200 million people infected worldwide.
Many areas of Brazil show low endemicity of schistosomiasis, and the current standard parasitological techniques are not sufficiently sensitive to detect the low-level helminth infections common in areas of low endemicity (ALEs).
This study compared the Kato-Katz (KK); Hoffman, Pons, and Janer (HH); enzyme-linked immunosorbent assay- (ELISA-) IgG and ELISA-IgM; indirect immunofluorescence technique (IFT-IgM); and qPCR techniques for schistosomiasis detection in serum and fecal samples, using the circumoval precipitin test (COPT) as reference.
An epidemiological survey was conducted in a randomized sample of residents from five neighborhoods of Barra Mansa, RJ, with 610 fecal and 612 serum samples.
ELISA-IgM (21.4%) showed the highest positivity and HH and KK techniques were the least sensitive (0.8%).
All techniques except qPCR-serum showed high accuracy (82–95.5%), differed significantly from COPT in positivity P<0.05, and showed poor agreement with COPT.
Medium agreement was seen with ELISA-IgG (Kappa = 0.377) and IFA (Kappa = 0.347).
Parasitological techniques showed much lower positivity rates than those by other techniques.
We suggest the possibility of using a combination of laboratory tools for the diagnosis of schistosomiasis in ALEs.
American Psychological Association (APA)
Espírito-Santo, Maria Cristina Carvalho& Alvarado-Mora, Mónica Viviana& Pinto, Pedro Luiz Silva& Sanchez, Maria Carmen Arroyo& Dias-Neto, Emmanuel& Castilho, Vera Lúcia Pagliusi…[et al.]. 2015. Comparative Study of the Accuracy of Different Techniques for the Laboratory Diagnosis of Schistosomiasis Mansoni in Areas of Low Endemicity in Barra Mansa City, Rio de Janeiro State, Brazil. BioMed Research International،Vol. 2015, no. 2015, pp.1-16.
https://search.emarefa.net/detail/BIM-1054316
Modern Language Association (MLA)
Espírito-Santo, Maria Cristina Carvalho…[et al.]. Comparative Study of the Accuracy of Different Techniques for the Laboratory Diagnosis of Schistosomiasis Mansoni in Areas of Low Endemicity in Barra Mansa City, Rio de Janeiro State, Brazil. BioMed Research International No. 2015 (2015), pp.1-16.
https://search.emarefa.net/detail/BIM-1054316
American Medical Association (AMA)
Espírito-Santo, Maria Cristina Carvalho& Alvarado-Mora, Mónica Viviana& Pinto, Pedro Luiz Silva& Sanchez, Maria Carmen Arroyo& Dias-Neto, Emmanuel& Castilho, Vera Lúcia Pagliusi…[et al.]. Comparative Study of the Accuracy of Different Techniques for the Laboratory Diagnosis of Schistosomiasis Mansoni in Areas of Low Endemicity in Barra Mansa City, Rio de Janeiro State, Brazil. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-16.
https://search.emarefa.net/detail/BIM-1054316
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054316