Sampling and Homogenization Strategies Significantly Influence the Detection of Foodborne Pathogens in Meat
Joint Authors
Rohde, Alexander
Hammerl, Jens Andre
Appel, Bernd
Dieckmann, Ralf
Al Dahouk, Sascha
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-11
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Efficient preparation of food samples, comprising sampling and homogenization, for microbiological testing is an essential, yet largely neglected, component of foodstuff control.
Salmonella enterica spiked chicken breasts were used as a surface contamination model whereas salami and meat paste acted as models of inner-matrix contamination.
A systematic comparison of different homogenization approaches, namely, stomaching, sonication, and milling by FastPrep-24 or SpeedMill, revealed that for surface contamination a broad range of sample pretreatment steps is applicable and loss of culturability due to the homogenization procedure is marginal.
In contrast, for inner-matrix contamination long treatments up to 8 min are required and only FastPrep-24 as a large-volume milling device produced consistently good recovery rates.
In addition, sampling of different regions of the spiked sausages showed that pathogens are not necessarily homogenously distributed throughout the entire matrix.
Instead, in meat paste the core region contained considerably more pathogens compared to the rim, whereas in the salamis the distribution was more even with an increased concentration within the intermediate region of the sausages.
Our results indicate that sampling and homogenization as integral parts of food microbiology and monitoring deserve more attention to further improve food safety.
American Psychological Association (APA)
Rohde, Alexander& Hammerl, Jens Andre& Appel, Bernd& Dieckmann, Ralf& Al Dahouk, Sascha. 2015. Sampling and Homogenization Strategies Significantly Influence the Detection of Foodborne Pathogens in Meat. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054356
Modern Language Association (MLA)
Rohde, Alexander…[et al.]. Sampling and Homogenization Strategies Significantly Influence the Detection of Foodborne Pathogens in Meat. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1054356
American Medical Association (AMA)
Rohde, Alexander& Hammerl, Jens Andre& Appel, Bernd& Dieckmann, Ralf& Al Dahouk, Sascha. Sampling and Homogenization Strategies Significantly Influence the Detection of Foodborne Pathogens in Meat. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054356
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054356