Genetic Variability of Candida albicans Sap8 Propeptide in Isolates from Different Types of Infection
Joint Authors
Carvalho-Pereira, Joana
Vaz, Catarina
Carneiro, Catarina
Pais, Célia
Sampaio, Paula
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-04
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The secreted aspartic proteases (Saps) are among the most studied virulence determinants in Candida albicans.
These proteins are translated as pre-pro-enzymes consisting of a signal sequence followed by a propeptide and the mature enzyme.
The propeptides of secreted proteinases are important for the correct processing, folding/secretion of the mature enzyme.
In this study, the DNA sequences of C.
albicans Saps were screened and a microsatellite was identified in SAP8 propeptide region.
The genetic variability of the repetitive region of Sap8 propeptide was determined in 108 C.
albicans independent strains isolated from different types of infection: oral infection (OI), oral commensal (OC), vulvovaginal candidiasis (VVC), and bloodstream infections (BSI).
Nine different propeptides for Sap8 processing were identified whose frequencies varied with the type of infection.
OC strains presented the highest gene diversity while OI isolated the lowest.
The contribution of the Saps to mucosal and systemic infections has been demonstrated and recently Sap8 has been implicated in the cleavage of a signalling glycoprotein that leads to Cek1-MAPK pathway activation.
This work is the first to identify a variable microsatellite in the propeptide of a secreted aspartic protease and brings new insights into the variability of Sap8.
American Psychological Association (APA)
Carvalho-Pereira, Joana& Vaz, Catarina& Carneiro, Catarina& Pais, Célia& Sampaio, Paula. 2015. Genetic Variability of Candida albicans Sap8 Propeptide in Isolates from Different Types of Infection. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054366
Modern Language Association (MLA)
Carvalho-Pereira, Joana…[et al.]. Genetic Variability of Candida albicans Sap8 Propeptide in Isolates from Different Types of Infection. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1054366
American Medical Association (AMA)
Carvalho-Pereira, Joana& Vaz, Catarina& Carneiro, Catarina& Pais, Célia& Sampaio, Paula. Genetic Variability of Candida albicans Sap8 Propeptide in Isolates from Different Types of Infection. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054366
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054366