Molecular Characterization of a New Alkaline-Tolerant Xylanase from Humicola insolens Y1
Joint Authors
Du, Yanlong
Yang, Hong
Huang, Huoqing
Zhang, Xiu
Wang, Yaru
Yao, Bin
Shi, Pengjun
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
An endo-1,4-β-xylanase-encoding gene, xyn11B, was cloned from the thermophilic fungus Humicola insolens Y1.
The gene encodes a multimodular xylanase that consists of a typical hydrophobic signal sequence, a catalytic domain of glycoside hydrolase (GH) family 11, a glycine-rich linker, and a family 1 carbohydrate binding module (CBM1).
Deduced Xyn11B shares the highest identity of 74% with a putative xylanase from Podospora anserina S mat+.
Recombinant Xyn11B was successfully expressed in Pichia pastoris and purified to electrophoretic homogeneity.
Xyn11B had a high specific activity of 382.0 U mg−1 towards beechwood xylan and showed optimal activity at pH 6.0 and 50°C.
Distinct from most reported acidic fungal xylanases, Xyn11B was alkaline-tolerant, retaining 30.7% of the maximal activity at pH 9.0.
The Km and Vmax values for beechwood xylan were 2.2 mg mL−1 and 462.8 μmol min−1 mg−1, respectively.
The enzyme exhibited a wider substrate specificity and produced a mixture of xylooligosaccharides.
All these favorable enzymatic properties make Xyn11B attractive for potential applications in various industries.
American Psychological Association (APA)
Shi, Pengjun& Du, Yanlong& Yang, Hong& Huang, Huoqing& Zhang, Xiu& Wang, Yaru…[et al.]. 2015. Molecular Characterization of a New Alkaline-Tolerant Xylanase from Humicola insolens Y1. BioMed Research International،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1054373
Modern Language Association (MLA)
Shi, Pengjun…[et al.]. Molecular Characterization of a New Alkaline-Tolerant Xylanase from Humicola insolens Y1. BioMed Research International No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1054373
American Medical Association (AMA)
Shi, Pengjun& Du, Yanlong& Yang, Hong& Huang, Huoqing& Zhang, Xiu& Wang, Yaru…[et al.]. Molecular Characterization of a New Alkaline-Tolerant Xylanase from Humicola insolens Y1. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1054373
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054373