Oxymatrine Downregulates HPV16E7 Expression and Inhibits Cell Proliferation in Laryngeal Squamous Cell Carcinoma Hep-2 Cells In Vitro
Joint Authors
Ying, Xin-Jiang
Jin, Bin
Chen, Xin-Wei
Xie, Jin
Xu, Hong-Ming
Dong, Pin
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Objective.
To investigate the possible mechanisms of oxymatrine’s role in anti laryngeal squamous cell carcinoma.
Methods.
We examined the effects of oxymatrine on the proliferation, cell cycle phase distribution, apoptosis, and the protein and mRNA expression levels of HPV16E7 gene in laryngeal carcinoma Hep-2 cells in vitro.
The HPV16E7 siRNA inhibition was also done to confirm the effect of downregulating HPV16E7 on the proliferation in Hep-2 cells.
Results.
Oxymatrine significantly inhibited the growth and proliferation of Hep-2 cells in a dose-dependence and time-dependence manner.
Oxymatrine blocked Hep-2 cells in G0/G1 phase, resulting in an obvious accumulation of G0/G1 phase cells while decreasing S phase cells.
Oxymatrine induced apoptosis of Hep-2 cells, whose apoptotic rate amounted to about 42% after treatment with 7 mg/mL oxymatrine for 72 h.
Oxymatrine also downregulated the expression of HPV16E7 gene, as determined by the western blotting and reverse transcription-polymerase chain reaction analysis.
Knockdown of HPV16E7 effectively inhibited the proliferation of Hep-2 cells.
Conclusions.
Oxymatrine inhibits the proliferation and induces apoptosis of laryngeal carcinoma Hep-2 cells, which might be mediated by a significant cell cycle arrest in G0/G1 phase and downregulation of HPV16E7 gene.
Oxymatrine is considered to be a likely preventive and curative candidate for laryngeal cancer.
American Psychological Association (APA)
Ying, Xin-Jiang& Jin, Bin& Chen, Xin-Wei& Xie, Jin& Xu, Hong-Ming& Dong, Pin. 2015. Oxymatrine Downregulates HPV16E7 Expression and Inhibits Cell Proliferation in Laryngeal Squamous Cell Carcinoma Hep-2 Cells In Vitro. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054378
Modern Language Association (MLA)
Ying, Xin-Jiang…[et al.]. Oxymatrine Downregulates HPV16E7 Expression and Inhibits Cell Proliferation in Laryngeal Squamous Cell Carcinoma Hep-2 Cells In Vitro. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1054378
American Medical Association (AMA)
Ying, Xin-Jiang& Jin, Bin& Chen, Xin-Wei& Xie, Jin& Xu, Hong-Ming& Dong, Pin. Oxymatrine Downregulates HPV16E7 Expression and Inhibits Cell Proliferation in Laryngeal Squamous Cell Carcinoma Hep-2 Cells In Vitro. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054378
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054378