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Effect of Clinoptilolite and Sepiolite Nanoclays on Human and Parasitic Highly Phagocytic Cells
Joint Authors
Carrero, Julio César
Laclette, Juan Pedro
Toledano-Magaña, Yanis
Flores-Santos, Leticia
Montes de Oca, Georgina
González-Montiel, Alfonso
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-27
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Nanoclays have potential applications in biomedicine raising the need to evaluate their toxicity in in vitro models as a first approach to its biocompatibility.
In this study, in vitro toxicity of clinoptilolite and sepiolite nanoclays (NC) was analyzed in highly phagocytic cultures of amoebas and human and mice macrophages.
While amebic viability was significantly affected only by sepiolite NC at concentrations higher than 0.1 mg/mL, the effect on macrophage cultures was dependent on the origin of the cells.
Macrophages derived from human peripheral blood monocytes were less affected in viability (25% decrease at 48 h), followed by the RAW 264.7 cell line (40%), and finally, macrophages derived from mice bone marrow monocytes (98%).
Moreover, the cell line and mice macrophages die mainly by necrosis, whereas human macrophages exhibit increased apoptosis.
Cytokine expression analysis in media of sepiolite NC treated cultures showed a proinflammatory profile (INFγ, IL-1α, IL-8, and IL-6), in contrast with clinoptilolite NC that induced lees cytokines with concomitant production of IL-10.
The results show that sepiolite NC is more toxic to amoebas and macrophages than clinoptilolite NC, mostly in a time and dose-dependent manner.
However, the effect of sepiolite NC was comparable with talc powder suggesting that both NC have low cytotoxicity in vitro.
American Psychological Association (APA)
Toledano-Magaña, Yanis& Flores-Santos, Leticia& Montes de Oca, Georgina& González-Montiel, Alfonso& Laclette, Juan Pedro& Carrero, Julio César. 2015. Effect of Clinoptilolite and Sepiolite Nanoclays on Human and Parasitic Highly Phagocytic Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1054442
Modern Language Association (MLA)
Toledano-Magaña, Yanis…[et al.]. Effect of Clinoptilolite and Sepiolite Nanoclays on Human and Parasitic Highly Phagocytic Cells. BioMed Research International No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1054442
American Medical Association (AMA)
Toledano-Magaña, Yanis& Flores-Santos, Leticia& Montes de Oca, Georgina& González-Montiel, Alfonso& Laclette, Juan Pedro& Carrero, Julio César. Effect of Clinoptilolite and Sepiolite Nanoclays on Human and Parasitic Highly Phagocytic Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1054442
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054442