In Vitro Antiproliferative Effect of the Acetone Extract of Rubus fairholmianus Gard. Root on Human Colorectal Cancer Cells
Joint Authors
Plackal Adimuriyil George, Blassan
Tynga, Ivan Mfouo
Abrahamse, H.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-21
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Plants and plant derived products exert chemopreventive effects on various cancer cell lines by the induction of cell death mechanisms.
The effects of root acetone extract of Rubus fairholmianus (RFRA) on the proliferation of human colorectal cancer (Caco-2) cells have been investigated in this study.
The extract led to a dose dependent decrease in both viability and proliferation and increased cytotoxicity using trypan blue exclusion, adenosine 5′-triphosphate (ATP), and lactate dehydrogenase (LDH) assay.
The morphological features of the treated cells were supportive for the antiproliferative activity.
The Annexin V/propidium iodide staining indicated that R.
fairholmianus induced toxic effects in Caco-2 cells and the percentages of the early and late apoptotic population significantly increased when compared with control cells.
Also we studied the apoptosis inducing ability of the extract by analysing caspase 3/7 activity and the induction of cell death via the effector caspases was confirmed; the activity increased in treated cells compared with control.
Thus the present findings highlight that the R.
fairholmianus root acetone extract exhibits antiproliferative activity on Caco-2 cells by the induction of apoptosis via caspase dependent pathway.
American Psychological Association (APA)
Plackal Adimuriyil George, Blassan& Tynga, Ivan Mfouo& Abrahamse, H.. 2015. In Vitro Antiproliferative Effect of the Acetone Extract of Rubus fairholmianus Gard. Root on Human Colorectal Cancer Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054444
Modern Language Association (MLA)
Plackal Adimuriyil George, Blassan…[et al.]. In Vitro Antiproliferative Effect of the Acetone Extract of Rubus fairholmianus Gard. Root on Human Colorectal Cancer Cells. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1054444
American Medical Association (AMA)
Plackal Adimuriyil George, Blassan& Tynga, Ivan Mfouo& Abrahamse, H.. In Vitro Antiproliferative Effect of the Acetone Extract of Rubus fairholmianus Gard. Root on Human Colorectal Cancer Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054444
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054444