Statistical Optimization of Laccase Production and Delignification of Sugarcane Bagasse by Pleurotus ostreatus in Solid-State Fermentation
Joint Authors
Karp, Susan Grace
Faraco, Vincenza
Amore, Antonella
Letti, Luiz Alberto Junior
Thomaz Soccol, Vanete
Soccol, Carlos Ricardo
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Laccases are oxidative enzymes related to the degradation of phenolic compounds, including lignin units, with concomitant reduction of oxygen to water.
Delignification is a necessary pretreatment step in the process of converting plant biomass into fermentable sugars.
The objective of this work was to optimize the production of laccases and to evaluate the delignification of sugarcane bagasse by Pleurotus ostreatus in solid-state fermentation.
Among eight variables (pH, water activity, temperature, and concentrations of CuSO4, (NH4)2SO4, KH2PO4, asparagine, and yeast extract), copper sulfate and ammonium sulfate concentrations were demonstrated to significantly influence laccase production.
The replacement of ammonium sulfate by yeast extract and the addition of ferulic acid as inducer provided increases of 5.7- and 2.0-fold, respectively, in laccase activity.
Optimization of laccase production as a function of yeast extract, copper sulfate, and ferulic acid concentrations was performed by response surface methodology and optimal concentrations were 6.4 g/L, 172.6 μM, and 1.86 mM, respectively.
Experimentally, the maximum laccase activity of 151.6 U/g was produced at the 5th day of solid-state fermentation.
Lignin content in sugarcane bagasse was reduced from 31.89% to 26.36% after 5 days and to 20.79% after 15 days by the biological treatment of solid-state fermentation.
American Psychological Association (APA)
Karp, Susan Grace& Faraco, Vincenza& Amore, Antonella& Letti, Luiz Alberto Junior& Thomaz Soccol, Vanete& Soccol, Carlos Ricardo. 2015. Statistical Optimization of Laccase Production and Delignification of Sugarcane Bagasse by Pleurotus ostreatus in Solid-State Fermentation. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054522
Modern Language Association (MLA)
Karp, Susan Grace…[et al.]. Statistical Optimization of Laccase Production and Delignification of Sugarcane Bagasse by Pleurotus ostreatus in Solid-State Fermentation. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1054522
American Medical Association (AMA)
Karp, Susan Grace& Faraco, Vincenza& Amore, Antonella& Letti, Luiz Alberto Junior& Thomaz Soccol, Vanete& Soccol, Carlos Ricardo. Statistical Optimization of Laccase Production and Delignification of Sugarcane Bagasse by Pleurotus ostreatus in Solid-State Fermentation. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054522
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054522