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Cytotoxicity of Cyclodipeptides from Pseudomonas aeruginosa PAO1 Leads to Apoptosis in Human Cancer Cell Lines
Joint Authors
Ochoa-Zarzosa, Alejandra
Guzmán-Rodríguez, Jaquelina Julia
Vázquez-Rivera, Dolores
González, Omar
Díaz-Pérez, Alma L.
López-Bucio, José
Meza-Carmen, Víctor
Campos-García, Jesús
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Pseudomonas aeruginosa is an opportunistic pathogen of plants and animals, which produces virulence factors in order to infect or colonize its eukaryotic hosts.
Cyclodipeptides (CDPs) produced by P.
aeruginosa exhibit cytotoxic properties toward human tumor cells.
In this study, we evaluated the effect of a CDP mix, comprised of cyclo(L-Pro-L-Tyr), cyclo(L-Pro-L-Val), and cyclo(L-Pro-L-Phe) that were isolated from P.
aeruginosa, on two human cancer cell lines.
Our results demonstrated that the CDP mix promoted cell death in cultures of the HeLa cervical adenocarcinoma and Caco-2 colorectal adenocarcinoma cell lines in a dose-dependent manner, with a 50% inhibitory concentration (IC50) of 0.53 and 0.66 mg/mL, for HeLa and Caco-2 cells, respectively.
Flow cytometric analysis, using annexin V and propidium iodide as apoptosis and necrosis indicators, respectively, clearly showed that HeLa and Caco-2 cells exhibited apoptotic characteristics when treated with the CDP mix at a concentration <0.001 mg/mL.
IC50 values for apoptotic cells in HeLa and Caco-2 cells were 6.5 × 10−5 and 1.8 × 10−4 mg/mL, respectively.
Our results indicate that an apoptotic pathway is involved in the inhibition of cell proliferation caused by the P.
aeruginosa CDP mix.
American Psychological Association (APA)
Vázquez-Rivera, Dolores& González, Omar& Guzmán-Rodríguez, Jaquelina Julia& Díaz-Pérez, Alma L.& Ochoa-Zarzosa, Alejandra& López-Bucio, José…[et al.]. 2015. Cytotoxicity of Cyclodipeptides from Pseudomonas aeruginosa PAO1 Leads to Apoptosis in Human Cancer Cell Lines. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1054600
Modern Language Association (MLA)
Vázquez-Rivera, Dolores…[et al.]. Cytotoxicity of Cyclodipeptides from Pseudomonas aeruginosa PAO1 Leads to Apoptosis in Human Cancer Cell Lines. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1054600
American Medical Association (AMA)
Vázquez-Rivera, Dolores& González, Omar& Guzmán-Rodríguez, Jaquelina Julia& Díaz-Pérez, Alma L.& Ochoa-Zarzosa, Alejandra& López-Bucio, José…[et al.]. Cytotoxicity of Cyclodipeptides from Pseudomonas aeruginosa PAO1 Leads to Apoptosis in Human Cancer Cell Lines. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1054600
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054600