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Effects of Arsenite Resistance on the Growth and Functional Gene Expression of Leptospirillum ferriphilum and Acidithiobacillus thiooxidans in Pure Culture and Coculture
Joint Authors
Liang, Yili
Jiang, Huidan
Yin, Huaqun
Xiao, Yunhua
Guo, Xue
Xu, Ying
Hu, Qi
Liu, Hongwei
Liu, Xueduan
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-12
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The response of iron-oxidizing Leptospirillum ferriphilum YSK and sulfur-oxidizing Acidithiobacillus thiooxidans A01 to arsenite under pure culture and coculture was investigated based on biochemical characterization (concentration of iron ion and pH value) and related gene expression.
L.
ferriphilum YSK and At.
thiooxidans A01 in pure culture could adapt up to 400 mM and 800 mM As(III) after domestication, respectively, although arsenite showed a negative effect on both strains.
The coculture showed a stronger sulfur and ferrous ion oxidation activity when exposed to arsenite.
In coculture, the pH value showed no significant difference when under 500 mM arsenite stress, and the cell number of At.
thiooxidans was higher than that in pure culture benefiting from the interaction with L.
ferriphilum.
The expression profile showed that the arsenic efflux system in the coculture was more active than that in pure culture, indicating that there is a synergetic interaction between At.
thiooxidans A01 and L.
ferriphilum YSK.
In addition, a model was proposed to illustrate the interaction between arsenite and the ars operon in L.
ferriphilum YSK and At.
thiooxidans A01.
This study will facilitate the effective application of coculture in the bioleaching process by taking advantage of strain-strain communication and coordination.
American Psychological Association (APA)
Jiang, Huidan& Liang, Yili& Yin, Huaqun& Xiao, Yunhua& Guo, Xue& Xu, Ying…[et al.]. 2015. Effects of Arsenite Resistance on the Growth and Functional Gene Expression of Leptospirillum ferriphilum and Acidithiobacillus thiooxidans in Pure Culture and Coculture. BioMed Research International،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1054618
Modern Language Association (MLA)
Jiang, Huidan…[et al.]. Effects of Arsenite Resistance on the Growth and Functional Gene Expression of Leptospirillum ferriphilum and Acidithiobacillus thiooxidans in Pure Culture and Coculture. BioMed Research International No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1054618
American Medical Association (AMA)
Jiang, Huidan& Liang, Yili& Yin, Huaqun& Xiao, Yunhua& Guo, Xue& Xu, Ying…[et al.]. Effects of Arsenite Resistance on the Growth and Functional Gene Expression of Leptospirillum ferriphilum and Acidithiobacillus thiooxidans in Pure Culture and Coculture. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1054618
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054618