![](/images/graphics-bg.png)
Delivery of bFGF for Tissue Engineering by Tethering to the ECM
Joint Authors
Suttinont, Chawapun
Mashimo, Yasumasa
Mie, Masayasu
Kobatake, Eiry
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-11
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Delivery of growth factors to target cells is an important subject in tissue engineering.
Towards that end, we have developed a growth factor-tethered extracellular matrix (ECM).
Here, basic fibroblast growth factor (bFGF) was tethered to extracellular matrix noncovalently.
The designed ECM was comprised of 12 repeats of the APGVGV peptide motif derived from elastin as a stable structural unit and included the well-known cell adhesive RGD peptide as an active functional unit.
To bind bFGF to the ECM, an acidic amino acid-rich sequence was introduced at the C-terminus of the ECM protein.
It consisted of 5 repeats of 4 aspartic acids and a serine, DDDDS.
bFGF has a highly basic amino acid domain.
Therefore, bFGF was tethered to the ECM protein by electrostatic interaction.
Cells cultured on bFGF-tethered ECM were well attached to the ECM and induced proliferation without addition of soluble bFGF.
American Psychological Association (APA)
Suttinont, Chawapun& Mashimo, Yasumasa& Mie, Masayasu& Kobatake, Eiry. 2015. Delivery of bFGF for Tissue Engineering by Tethering to the ECM. BioMed Research International،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1054637
Modern Language Association (MLA)
Suttinont, Chawapun…[et al.]. Delivery of bFGF for Tissue Engineering by Tethering to the ECM. BioMed Research International No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1054637
American Medical Association (AMA)
Suttinont, Chawapun& Mashimo, Yasumasa& Mie, Masayasu& Kobatake, Eiry. Delivery of bFGF for Tissue Engineering by Tethering to the ECM. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1054637
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054637