Sequence Variation in HSP40 Gene among 16 Toxoplasma gondii Isolates from Different Hosts and Geographical Locations
Joint Authors
Zhu, Xing-Quan
Li, Zhong-Yuan
Lu, Jing
Zhou, Dong-Hui
Chen, Jia
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-03
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Toxoplasma gondii with worldwide distribution has received substantial medical and scientific attentions as it causes serious clinical and veterinary problems especially for pregnant women and immunocompromised patients.
Heat shock protein 40 (HSP40) plays a variety of essential roles in the pathogenesis of this protozoan parasite.
In order to detail the genetic diversity of HSP40 gene, 16 T.
gondii strains from different hosts and geographical locations were used in this study.
Our results showed that HSP40 sequence of the examined strains was between 6621 bp and 6644 bp in length, and their A+T content was from 48.54% to 48.80%.
Furthermore, sequence analysis presented 195 nucleotide mutation positions (0.12%–1.14%) including 29 positions in CDS (0.02%–0.12%) compared with T.
gondii ME49 strain (ToxoDB: TGME49_265310).
Phylogenetic assay revealed that T.
gondii strains representing three classical genotypes (Types I, II, and III) were completely separated into different clusters by maximum parsimony (MP) method, but Type II and ToxoDB#9 strains were grouped into the same cluster.
These results suggested that HSP40 gene is not a suitable marker for T.
gondii population genetic research, though three classical genotypes of T.
gondii could be differentiated by restriction enzymes MscI and EarI existing in amplicon C.
American Psychological Association (APA)
Li, Zhong-Yuan& Lu, Jing& Zhou, Dong-Hui& Chen, Jia& Zhu, Xing-Quan. 2015. Sequence Variation in HSP40 Gene among 16 Toxoplasma gondii Isolates from Different Hosts and Geographical Locations. BioMed Research International،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1054643
Modern Language Association (MLA)
Li, Zhong-Yuan…[et al.]. Sequence Variation in HSP40 Gene among 16 Toxoplasma gondii Isolates from Different Hosts and Geographical Locations. BioMed Research International No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1054643
American Medical Association (AMA)
Li, Zhong-Yuan& Lu, Jing& Zhou, Dong-Hui& Chen, Jia& Zhu, Xing-Quan. Sequence Variation in HSP40 Gene among 16 Toxoplasma gondii Isolates from Different Hosts and Geographical Locations. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1054643
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054643