Bone Regeneration of HydroxyapatiteAlumina Bilayered Scaffold with 3 mm Passage-Like Medullary Canal in Canine Tibia Model

Abstract EN

The aim of this study was to evaluate the bone regeneration of hydroxyapatite (HA)/alumina bilayered scaffold with a 3 mm passage-like medullary canal in a beagle tibia model.

A porous HA/alumina scaffold was fabricated using a polymeric template-coating technique.

HA/alumina scaffold dimensions were 10 mm in outer diameter, 20 mm in length, and with either a 3 mm passage or no passage.

A 20 mm segmental defect was induced using an oscillating saw through the diaphysis of the beagle tibia.

The defects of six beagles were filled with HA/alumina bilayered scaffolds with a 3 mm passage or without.

The segmental defect was fixated using one bone plate and six screws.

Bone regeneration within the HA/alumina scaffolds was observed at eight weeks after implantation.

The evaluation of bone regeneration within the scaffolds after implantation in a beagle tibia was performed using radiography, computerized tomography (CT), micro-CT, and fluorescence microscopy.

New bone successfully formed in the tibia defects treated with 3 mm passage HA/alumina scaffolds compared to without-passage HA/alumina scaffolds.

It was concluded that the HA/alumina bilayered scaffold with 3 mm passage-like medullary canal was instrumental in inducing host-scaffold engraftment of the defect as well as distributing the newly formed bone throughout the scaffold at 8 weeks after implantation.