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The Benefit of a Human Bone Marrow Stem Cells Concentrate in addition to an Inorganic Scaffold for Bone Regeneration: An In Vitro Study
Joint Authors
Lopes, M. Ascenção
Cabral, A. T.
Santos, J. Domingos
Gutierres, Manuel
Lopes, A.
Fernandes, M. H.
Monteiro, E.
Torres, Joao
van Eck, Carola
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-22
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
This work compares the osteoblastic behaviour of a bone marrow (BM) aspirate and a prepared BM concentrate of nucleated cells associated with a glass reinforced hydroxyapatite composite (GRHC) in a microporous pellet formulation.
Methods.
BM aspirate (30 mL) was collected during 3 orthopedic surgical procedures, and a concentration system was used to achieve 3 rapid preparations of a concentrate of nucleated cells (3 mL) from the BM aspirates.
The BM aspirates (53% cell viability; 2.7×106 nucleated cell/mL) and the BM concentrates (76% cell viability; 2×107 nucleated cell/mL) were cultured over glass reinforced hydroxyapatite pellets, at the same volume/mass ratio, for 30 days.
Cultures performed in standard tissue culture plates were used as control.
Results.
The colonized BM concentrate/material constructs exhibited a representative osteoblastic proliferation/differentiation pathway, evidenced by a high alkaline phosphatase (ALP) activity, expression of collagen type 1, ALP, BMP-2, M-CSF, RANKL, and OPG, and formation of a calcium phosphate mineralized matrix.
A clear improved behaviour was noticed compared to the BM aspirate/material constructs.
Conclusions.
The results suggest the benefit of using an autologous BM concentrate/material construct in the clinical setting, in bone regeneration applications.
American Psychological Association (APA)
Torres, Joao& Lopes, A.& Lopes, M. Ascenção& Gutierres, Manuel& Cabral, A. T.& Fernandes, M. H.…[et al.]. 2015. The Benefit of a Human Bone Marrow Stem Cells Concentrate in addition to an Inorganic Scaffold for Bone Regeneration: An In Vitro Study. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1054716
Modern Language Association (MLA)
Torres, Joao…[et al.]. The Benefit of a Human Bone Marrow Stem Cells Concentrate in addition to an Inorganic Scaffold for Bone Regeneration: An In Vitro Study. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1054716
American Medical Association (AMA)
Torres, Joao& Lopes, A.& Lopes, M. Ascenção& Gutierres, Manuel& Cabral, A. T.& Fernandes, M. H.…[et al.]. The Benefit of a Human Bone Marrow Stem Cells Concentrate in addition to an Inorganic Scaffold for Bone Regeneration: An In Vitro Study. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1054716
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054716