Downregulation of Heparanase Expression Results in Suppression of Invasion, Migration, and Adhesion Abilities of Hepatocellular Carcinoma Cells
Joint Authors
Chen, Xiao-Peng
Luo, Jun-Sheng
Tian, Ye
Nie, Chen-Lin
Cui, Wei
Zhang, Wei-Dong
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-29
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Objective.
Heparanase (HPSE) is high-expressed in most malignant tumors including hepatocellular carcinoma (HCC) and promotes cancer cell invasion and migration.
The aim of the study is to explore whether HPSE enhances adhesion in metastasis of HCC cells.
Methods.
HPSE expressions in human HCC cells were measured with real-time RT-PCR and Western blot analysis.
Four recombinant miRNA vectors pcDNATM6.2-GW/EmGFP-miR-HPSE (pmiR-HPSE) were transfected into HCCLM3 cell.
HPSE expression in transfected cell was measured.
The cell invasion, migration, and adhesion abilities were detected, respectively.
Results.
Both HPSE mRNA and protein relative expression levels were higher in HepG2, BEL-7402, and HCCLM3 cells than those in normal hepatocyte ( P < 0.05 ).
HPSE showed highest expression level in HCCLM3 cell ( P < 0.05 ).
Transfection efficiencies of four miRNA vectors were 75%–85%.
The recombinant vectors significantly decreased HPSE expression in transfected HCCLM3 cells ( P < 0.01 ), and pmiR-HPSE-1 showed best interference effect ( P < 0.05 ).
pmiR-HPSE-1 significantly decreased the penetrated and migrating cells numbers and adherence rate of HCCLM3 cells ( P < 0.0 5 ).
Conclusion.
HPSE is a potentiator of cell adhesion in metastasis of HCC.
American Psychological Association (APA)
Chen, Xiao-Peng& Luo, Jun-Sheng& Tian, Ye& Nie, Chen-Lin& Cui, Wei& Zhang, Wei-Dong. 2015. Downregulation of Heparanase Expression Results in Suppression of Invasion, Migration, and Adhesion Abilities of Hepatocellular Carcinoma Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1054722
Modern Language Association (MLA)
Chen, Xiao-Peng…[et al.]. Downregulation of Heparanase Expression Results in Suppression of Invasion, Migration, and Adhesion Abilities of Hepatocellular Carcinoma Cells. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1054722
American Medical Association (AMA)
Chen, Xiao-Peng& Luo, Jun-Sheng& Tian, Ye& Nie, Chen-Lin& Cui, Wei& Zhang, Wei-Dong. Downregulation of Heparanase Expression Results in Suppression of Invasion, Migration, and Adhesion Abilities of Hepatocellular Carcinoma Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1054722
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054722