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Application of FTIR-ATR Spectroscopy to Determine the Extent of Lipid Peroxidation in Plasma during Haemodialysis
Joint Authors
Oleszko, Adam
Olsztyńska-Janus, Sylwia
Grzeszczuk-Kuć, Karolina
Bujok, Jolanta
Gałecka, Katarzyna
Czerski, Albert
Komorowska, Małgorzata
Walski, Tomasz
Witkiewicz, Wojciech
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-19
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
During a haemodialysis (HD), because of the contact of blood with the surface of the dialyser, the immune system becomes activated and reactive oxygen species (ROS) are released into plasma.
Particularly exposed to the ROS are lipids and proteins contained in plasma, which undergo peroxidation.
The main breakdown product of oxidized lipids is the malondialdehyde (MDA).
A common method for measuring the concentration of MDA is a thiobarbituric acid reactive substances (TBARS) method.
Despite the formation of MDA in plasma during HD, its concentration decreases because it is removed from the blood in the dialyser.
Therefore, this research proposes the Fourier Transform Infrared Attenuated Total Reflectance (FTIR-ATR) spectroscopy, which enables determination of primary peroxidation products.
We examined the influence of the amount of hydrogen peroxide added to lipid suspension that was earlier extracted from plasma specimen on lipid peroxidation with use of TBARS and FTIR-ATR methods.
Linear correlation between these methods was shown.
The proposed method was effective during the evaluation of changes in the extent of lipid peroxidation in plasma during a haemodialysis in sheep.
A measurement using the FTIR-ATR showed an increase in plasma lipid peroxidation after 15 and 240 minutes of treatment, while the TBARS concentration was respectively lower.
American Psychological Association (APA)
Oleszko, Adam& Olsztyńska-Janus, Sylwia& Walski, Tomasz& Grzeszczuk-Kuć, Karolina& Bujok, Jolanta& Gałecka, Katarzyna…[et al.]. 2015. Application of FTIR-ATR Spectroscopy to Determine the Extent of Lipid Peroxidation in Plasma during Haemodialysis. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054736
Modern Language Association (MLA)
Oleszko, Adam…[et al.]. Application of FTIR-ATR Spectroscopy to Determine the Extent of Lipid Peroxidation in Plasma during Haemodialysis. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1054736
American Medical Association (AMA)
Oleszko, Adam& Olsztyńska-Janus, Sylwia& Walski, Tomasz& Grzeszczuk-Kuć, Karolina& Bujok, Jolanta& Gałecka, Katarzyna…[et al.]. Application of FTIR-ATR Spectroscopy to Determine the Extent of Lipid Peroxidation in Plasma during Haemodialysis. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054736
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054736