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Evaluation of Hemagglutination Activity of Chitosan Nanoparticles Using Human Erythrocytes
Joint Authors
Castellano, Lúcio Roberto
Medeiros, Eliton S.
Lima, Jefferson Muniz de
Sarmento, Ronaldo Rodrigues
Souza, Joelma Rodrigues de
Brayner, Fábio André
Feitosa, Ana Paula Sampaio
Padilha, Rafael
Alves, Luiz Carlos
Porto, Isaque Jerônimo
Batista, Roberta Ferreti Bonan Dantas
Bonan, Paulo Rogério Ferreti
Oliveira, Juliano E.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-11
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Chitosan is a polysaccharide composed of randomly distributed chains of β-(1-4) D-glucosamine and N-acetyl-D-glucosamine.
This compound is obtained by partial or total deacetylation of chitin in acidic solution.
The chitosan-based hemostatic agents have been gaining much attention in the management of bleeding.
The aim of this study was to evaluate in vitro hemagglutination activity of chitosan nanoparticles using human erythrocytes.
The preparation of nanoparticles was achieved by ionotropic gelification technique followed by neutralization with NaOH 1 mol/L−1.
The hemagglutination activity was performed on a solution of 2% erythrocytes (pH 7.4 on PBS) collected from five healthy volunteers.
The hemolysis determination was made by spectrophotometric analysis.
Chitosan nanoparticle solutions without NaOH addition changed the reddish colour of the wells into brown, suggesting an oxidative reaction of hemoglobin and possible cell lysis.
All neutralized solutions of chitosan nanoparticles presented positive haemagglutination, without any change in reaction color.
Chitosan nanoparticles presented hemolytic activity ranging from 186.20 to 223.12%, while neutralized solutions ranged from 2.56 to 72.54%, comparing to distilled water.
Results highlight the need for development of new routes of synthesis of chitosan nanoparticles within human physiologic pH.
American Psychological Association (APA)
Lima, Jefferson Muniz de& Sarmento, Ronaldo Rodrigues& Souza, Joelma Rodrigues de& Brayner, Fábio André& Feitosa, Ana Paula Sampaio& Padilha, Rafael…[et al.]. 2015. Evaluation of Hemagglutination Activity of Chitosan Nanoparticles Using Human Erythrocytes. BioMed Research International،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1054743
Modern Language Association (MLA)
Lima, Jefferson Muniz de…[et al.]. Evaluation of Hemagglutination Activity of Chitosan Nanoparticles Using Human Erythrocytes. BioMed Research International No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1054743
American Medical Association (AMA)
Lima, Jefferson Muniz de& Sarmento, Ronaldo Rodrigues& Souza, Joelma Rodrigues de& Brayner, Fábio André& Feitosa, Ana Paula Sampaio& Padilha, Rafael…[et al.]. Evaluation of Hemagglutination Activity of Chitosan Nanoparticles Using Human Erythrocytes. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1054743
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054743