Acupuncture May Exert Its Therapeutic Effect through MicroRNA-339Sirt2NF κ BFOXO1 Axis
Joint Authors
Zhou, Shu-Feng
Wang, Jia-You
Li, Hui
Ma, Chun-Mei
Wang, Jia-Lu
Lai, Xin-Sheng
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-28
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Recently, we have found that a number of microRNAs (miRNAs) and proteins are involved in the response to acupuncture therapy in hypertensive rats.
Our bioinformatics study suggests an association between these miRNAs and proteins, which include miR-339 and sirtuin 2 (Sirt2).
In this paper, we aimed to investigate whether Sirt2 was a direct target of miR-339 in neurons.
In human SH-SY5Y cells, the luciferase assay implied that Sirt2 was likely a target of miRNA-339.
Overexpression of miR-339 downregulated Sirt2 expression, while knockdown of miR-339 upregulated Sirt2 expression in human SH-SY5Y cells and rat PC12 cells.
In addition, overexpression of miR-399 increased the acetylation of nuclear factor- κ B (NF- κ B) and forkhead box protein O1 (FOXO1) in SH-SY5Y cells, which are known targets of Sirt2.
Our findings demonstrate that miR-339 regulates Sirt2 in human and rat neurons.
Since Sirt2 plays a critical role in multiple important cellular functions, our data imply that acupuncture may act through epigenetic changes and subsequent action on their targets, such as miRNA-339/Sirt2/NF- κ B/FOXO1 axis.
Some physiological level changes of neurons after altering the miR-339 levels are needed to validate the suggested therapeutic role of miR-339/Sirt2/NF- κ B/FOXO1 axis in response to acupuncture therapy in the future work.
American Psychological Association (APA)
Wang, Jia-You& Li, Hui& Ma, Chun-Mei& Wang, Jia-Lu& Lai, Xin-Sheng& Zhou, Shu-Feng. 2015. Acupuncture May Exert Its Therapeutic Effect through MicroRNA-339Sirt2NF κ BFOXO1 Axis. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1054749
Modern Language Association (MLA)
Wang, Jia-You…[et al.]. Acupuncture May Exert Its Therapeutic Effect through MicroRNA-339Sirt2NF κ BFOXO1 Axis. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1054749
American Medical Association (AMA)
Wang, Jia-You& Li, Hui& Ma, Chun-Mei& Wang, Jia-Lu& Lai, Xin-Sheng& Zhou, Shu-Feng. Acupuncture May Exert Its Therapeutic Effect through MicroRNA-339Sirt2NF κ BFOXO1 Axis. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1054749
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054749