Molecular Analysis of AFP and HSA Interactions with PTEN Protein
Joint Authors
Lin, Bo
Zhu, Mingyue
Zhou, Peng
Li, Mengsen
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-20
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Human cytoplasmic alpha-fetoprotein (AFP) has been classified as a member of the albuminoid gene family.
The protein sequence of AFP has significant homology to that of human serum albumin (HSA), but its biological characteristics are vastly different from HSA.
The AFP functions as a regulator in the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) pathway, but HSA plays a key role as a transport protein.
To probe their molecular mechanisms, we have applied colocalization, coimmunoprecipitation (co-IP), and molecular docking approaches to analyze the differences between AFP and HSA.
The data from colocalization and co-IP displayed a strong interaction between AFP and PTEN (phosphatase and tensin homolog), demonstrating that AFP did bind to PTEN, but HSA did not.
The molecular docking study further showed that the AFP domains I and III could contact with PTEN.
In silicon substitutions of AFP binding site residues at position 490M/K and 105L/R corresponding to residues K490 and R105 in HSA resulted in steric clashes with PTEN residues R150 and K46, respectively.
These steric clashes may explain the reason why HSA cannot bind to PTEN.
Ultimately, the experimental results and the molecular modeling data from the interactions of AFP and HSA with PTEN will help us to identify targets for designing drugs and vaccines against human hepatocellular carcinoma.
American Psychological Association (APA)
Zhu, Mingyue& Lin, Bo& Zhou, Peng& Li, Mengsen. 2015. Molecular Analysis of AFP and HSA Interactions with PTEN Protein. BioMed Research International،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1054775
Modern Language Association (MLA)
Zhu, Mingyue…[et al.]. Molecular Analysis of AFP and HSA Interactions with PTEN Protein. BioMed Research International No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1054775
American Medical Association (AMA)
Zhu, Mingyue& Lin, Bo& Zhou, Peng& Li, Mengsen. Molecular Analysis of AFP and HSA Interactions with PTEN Protein. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1054775
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054775