Oxidative Stress Alters miRNA and Gene Expression Profiles in Villous First Trimester Trophoblasts
Joint Authors
Abdel-Rahman, S. Z.
Cross, Courtney E.
Tolba, Mai F.
Rondelli, Catherine M.
Xu, Meixiang
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-03
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The relationship between oxidative stress and miRNA changes in placenta as a potential mechanism involved in preeclampsia (PE) is not fully elucidated.
We investigated the impact of oxidative stress on miRNAs and mRNA expression profiles of genes associated with PE in villous 3A first trimester trophoblast cells exposed to H2O2 at 12 different concentrations (0-1 mM) for 0.5, 4, 24, and 48 h.
Cytotoxicity, determined using the SRB assay, was used to calculate the IC50 of H2O2.
RNA was extracted after 4 h exposure to H2O2 for miRNA and gene expression profiling.
H2O2 exerted a concentration- and time-dependent cytotoxicity on 3A trophoblast cells.
Short-term exposure of 3A cells to low concentration of H2O2 (5% of IC50) significantly altered miRNA profile as evidenced by significant changes in 195 out of 595 evaluable miRNAs.
Tool for annotations of microRNAs (TAM) analysis indicated that these altered miRNAs fall into 43 clusters and 34 families, with 41 functions identified.
Exposure to H2O2 altered mRNA expression of 22 out of 84 key genes involved in dysregulation of placental development.
In conclusion, short-term exposure of villous first trimester trophoblasts to low concentrations of H2O2 significantly alters miRNA profile and expression of genes implicated in placental development.
American Psychological Association (APA)
Cross, Courtney E.& Tolba, Mai F.& Rondelli, Catherine M.& Xu, Meixiang& Abdel-Rahman, S. Z.. 2015. Oxidative Stress Alters miRNA and Gene Expression Profiles in Villous First Trimester Trophoblasts. BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1054777
Modern Language Association (MLA)
Cross, Courtney E.…[et al.]. Oxidative Stress Alters miRNA and Gene Expression Profiles in Villous First Trimester Trophoblasts. BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1054777
American Medical Association (AMA)
Cross, Courtney E.& Tolba, Mai F.& Rondelli, Catherine M.& Xu, Meixiang& Abdel-Rahman, S. Z.. Oxidative Stress Alters miRNA and Gene Expression Profiles in Villous First Trimester Trophoblasts. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1054777
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054777