Investigation of Ser315 Substitutions within katG Gene in Isoniazid-Resistant Clinical Isolates of Mycobacterium tuberculosis from South India
Joint Authors
Unissa, A. Nusrath
Selvakumar, N.
Narayanan, Sujatha
Suganthi, C.
Hanna, L. E.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-28
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Mutation at codon 315 of katG gene is the major cause for isoniazid (INH) resistance in Mycobacterium tuberculosis (M.
tuberculosis).
Substitution at codon 315 of katG gene was analyzed in 85 phenotypically resistant isolates collected from various parts of southern India by direct sequencing method.
The obtained results were interpreted in the context of minimum inhibitory concentration (MIC) of INH.
Of the 85 phenotypically resistant isolates, 56 (66%) were also correlated by the presence of resistance mutations in the katG gene; 47 of these isolates had ACC, 6 had AAC, 2 had ATC, and one had CGC codon.
The frequency of Ser315 substitution in katG gene was found to be higher (70%) amongst multidrug-resistant (MDR) strains than among non-MDR (61%) INH-resistant isolates.
Further, the frequency of mutations was found to be greater (74%) in isolates with higher MIC values in contrast to those isolates with low MIC values (58%).
Therefore, the study identified high prevalence of Ser315Thr substitution in katG gene of INH-resistant isolates from south India.
Also, isolates harboring this substitution were found to be associated with multidrug and high level INH resistance.
American Psychological Association (APA)
Unissa, A. Nusrath& Selvakumar, N.& Narayanan, Sujatha& Suganthi, C.& Hanna, L. E.. 2015. Investigation of Ser315 Substitutions within katG Gene in Isoniazid-Resistant Clinical Isolates of Mycobacterium tuberculosis from South India. BioMed Research International،Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1054783
Modern Language Association (MLA)
Unissa, A. Nusrath…[et al.]. Investigation of Ser315 Substitutions within katG Gene in Isoniazid-Resistant Clinical Isolates of Mycobacterium tuberculosis from South India. BioMed Research International No. 2015 (2015), pp.1-5.
https://search.emarefa.net/detail/BIM-1054783
American Medical Association (AMA)
Unissa, A. Nusrath& Selvakumar, N.& Narayanan, Sujatha& Suganthi, C.& Hanna, L. E.. Investigation of Ser315 Substitutions within katG Gene in Isoniazid-Resistant Clinical Isolates of Mycobacterium tuberculosis from South India. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1054783
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054783