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Analysis of the Expression and Polymorphism of APOE, HSP, BDNF, and GRIN2B Genes Associated with the Neurodegeneration Process in the Pathogenesis of Primary Open Angle Glaucoma
Joint Authors
Szaflik, Jerzy
Nowak, Alicja
Majsterek, Ireneusz
Przybyłowska-Sygut, Karolina
Pytel, Dariusz
Szymanek, Katarzyna
Szaflik, Jacek P.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-29
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Glaucoma is characterized by optic neuropathy of the RGC or retinal nerve fiber.
The aim of this study was to evaluate a relationship between the neurodegenerative genes’ polymorphisms of the APOE (rs449647), BDNF (rs2030324), GRIN2B (rs3764028), and HSP70-1 (rs1043618) and the occurrence risk of POAG and to investigate its effect on allele-specific gene expression.
Genomic DNA was extracted from peripheral blood.
Analysis of the genes’ polymorphisms was performed using PCR-RFLP.
The level of mRNA expression was determined by QRT-PCR.
We showed a statistically significant association of BDNF and APOE genes’ polymorphisms with a risk of POAG occurrence.
There was a statistically significant association of the rs2030324 polymorphism with progression of POAG based on cup disc ratio value and rs1043618 polymorphism based on nerve fiber index and rim area.
Furthermore, we found that mean HSP70-1 mRNA expression was significantly lower in the case of individuals with the G/G genotype than in the case of minor allele carriers, that is, G/C and C/C.
We also found that BDNF and HSP70-1 expression level are associated with the progression of POAG based on rim area value.
In conclusion, our results suggest that BDNF, APOE, and HSP70-1 genes might be associated with a risk of POAG occurrence in the Polish population.
American Psychological Association (APA)
Nowak, Alicja& Majsterek, Ireneusz& Przybyłowska-Sygut, Karolina& Pytel, Dariusz& Szymanek, Katarzyna& Szaflik, Jerzy…[et al.]. 2015. Analysis of the Expression and Polymorphism of APOE, HSP, BDNF, and GRIN2B Genes Associated with the Neurodegeneration Process in the Pathogenesis of Primary Open Angle Glaucoma. BioMed Research International،Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1054786
Modern Language Association (MLA)
Nowak, Alicja…[et al.]. Analysis of the Expression and Polymorphism of APOE, HSP, BDNF, and GRIN2B Genes Associated with the Neurodegeneration Process in the Pathogenesis of Primary Open Angle Glaucoma. BioMed Research International No. 2015 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1054786
American Medical Association (AMA)
Nowak, Alicja& Majsterek, Ireneusz& Przybyłowska-Sygut, Karolina& Pytel, Dariusz& Szymanek, Katarzyna& Szaflik, Jerzy…[et al.]. Analysis of the Expression and Polymorphism of APOE, HSP, BDNF, and GRIN2B Genes Associated with the Neurodegeneration Process in the Pathogenesis of Primary Open Angle Glaucoma. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1054786
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054786