Bone Regeneration by NanohydroxyapatiteChitosanPoly(lactide-co-glycolide) Scaffolds Seeded with Human Umbilical Cord Mesenchymal Stem Cells in the Calvarial Defects of the Nude Mice
Joint Authors
Wang, Fei
Su, Xiao-Xia
Guo, Yu-Cheng
Li, Ang
Zhang, Yin-Cheng
Zhou, Hong
Qiao, Hu
Guan, Li-Min
Zou, Min
Si, Xin-Qin
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
In the preliminary study, we have found an excellent osteogenic property of nanohydroxyapatite/chitosan/poly(lactide-co-glycolide) (nHA/CS/PLGA) scaffolds seeded with human umbilical cord mesenchymal stem cells (hUCMSCs) in vitro and subcutaneously in the nude mice.
The aim of this study was to further evaluate the osteogenic capacity of nHA/CS/PLGA scaffolds seeded with hUCMSCs in the calvarial defects of the nude mice.
Totally 108 nude mice were included and divided into 6 groups: PLGA scaffolds + hUCMSCs; nHA/PLGA scaffolds + hUCMSCs; CS/PLGA scaffolds + hUCMSCs; nHA/CS/PLGA scaffolds + hUCMSCs; nHA/CS/PLGA scaffolds without seeding; the control group (no scaffolds) ( n = 18 ).
The scaffolds were implanted into the calvarial defects of nude mice.
The amount of new bones was evaluated by fluorescence labeling, H&E staining, and Van Gieson staining at 4 and 8 weeks, respectively.
The results demonstrated that the amount of new bones was significantly increased in the group of nHA/CS/PLGA scaffolds seeded with hUCMSCs ( p < 0.01 ).
On the basis of previous studies in vitro and in subcutaneous implantation of the nude mice, the results revealed that the nHA and CS also enhanced the bone regeneration by nHA/CS/PLGA scaffolds seeded with hUCMSCs in the calvarial defects of the nude mice at early stage.
American Psychological Association (APA)
Wang, Fei& Su, Xiao-Xia& Guo, Yu-Cheng& Li, Ang& Zhang, Yin-Cheng& Zhou, Hong…[et al.]. 2015. Bone Regeneration by NanohydroxyapatiteChitosanPoly(lactide-co-glycolide) Scaffolds Seeded with Human Umbilical Cord Mesenchymal Stem Cells in the Calvarial Defects of the Nude Mice. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1054805
Modern Language Association (MLA)
Wang, Fei…[et al.]. Bone Regeneration by NanohydroxyapatiteChitosanPoly(lactide-co-glycolide) Scaffolds Seeded with Human Umbilical Cord Mesenchymal Stem Cells in the Calvarial Defects of the Nude Mice. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1054805
American Medical Association (AMA)
Wang, Fei& Su, Xiao-Xia& Guo, Yu-Cheng& Li, Ang& Zhang, Yin-Cheng& Zhou, Hong…[et al.]. Bone Regeneration by NanohydroxyapatiteChitosanPoly(lactide-co-glycolide) Scaffolds Seeded with Human Umbilical Cord Mesenchymal Stem Cells in the Calvarial Defects of the Nude Mice. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1054805
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054805