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A MicroRNA-Transcription Factor Blueprint for Early Atrial Arrhythmogenic Remodeling
Joint Authors
Torrado, Mario
Lozano-Velasco, Estefanía
Hernández-Torres, Francisco
Calviño, Ramón
Aldama, Guillermo
Centeno, Alberto
Castro-Beiras, Alfonso
Mikhailov, Alexander T.
Franco, Diego
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-28
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Spontaneous self-terminating atrial fibrillation (AF) is one of the most common heart rhythm disorders, yet the regulatory molecular mechanisms underlying this syndrome are rather unclear.
MicroRNA (miRNA) transcriptome and expression of candidate transcription factors (TFs) with potential roles in arrhythmogenesis, such as Pitx2, Tbx5, and myocardin (Myocd), were analyzed by microarray, qRT-PCR, and Western blotting in left atrial (LA) samples from pigs with transitory AF established by right atrial tachypacing.
Induced ectopic tachyarrhythmia caused rapid and substantial miRNA remodeling associated with a marked downregulation of Pitx2, Tbx5, and Myocd expression in atrial myocardium.
The downregulation of Pitx2, Tbx5, and Myocd was inversely correlated with upregulation of the corresponding targeting miRNAs (miR-21, miR-10a/10b, and miR-1, resp.) in the LA of paced animals.
Through in vitro transient transfections of HL-1 atrial myocytes, we further showed that upregulation of miR-21 did result in downregulation of Pitx2 in cardiomyocyte background.
The results suggest that immediate-early miRNA remodeling coupled with deregulation of TF expression underlies the onset of AF.
American Psychological Association (APA)
Torrado, Mario& Franco, Diego& Lozano-Velasco, Estefanía& Hernández-Torres, Francisco& Calviño, Ramón& Aldama, Guillermo…[et al.]. 2015. A MicroRNA-Transcription Factor Blueprint for Early Atrial Arrhythmogenic Remodeling. BioMed Research International،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1054807
Modern Language Association (MLA)
Torrado, Mario…[et al.]. A MicroRNA-Transcription Factor Blueprint for Early Atrial Arrhythmogenic Remodeling. BioMed Research International No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1054807
American Medical Association (AMA)
Torrado, Mario& Franco, Diego& Lozano-Velasco, Estefanía& Hernández-Torres, Francisco& Calviño, Ramón& Aldama, Guillermo…[et al.]. A MicroRNA-Transcription Factor Blueprint for Early Atrial Arrhythmogenic Remodeling. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1054807
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054807