Sevoflurane Induces DNA Damage Whereas Isoflurane Leads to Higher Antioxidative Status in Anesthetized Rats
Joint Authors
Rocha, Thalita L. A.
Dias-Junior, Carlos A.
Possomato-Vieira, Jose S.
Gonçalves-Rizzi, Victor H.
Nogueira, Flávia R.
de Souza, Kátina M.
Braz, Leandro G.
Braz, Mariana G.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-25
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Taking into account that there are controversial antioxidative effects of inhalational anesthetics isoflurane and sevoflurane and absence of comparison of genotoxicity of both anesthetics in animal model, the aim of this study was to compare DNA damage and antioxidant status in Wistar rats exposed to a single time to isoflurane or sevoflurane.
The alkaline single-cell gel electrophoresis assay (comet assay) was performed in order to evaluate DNA damage in whole blood cells of control animals (unexposed; n = 6) and those exposed to 2% isoflurane (n = 6) or 4% sevoflurane (n = 6) for 120 min.
Plasma antioxidant status was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
There was no statistically significant difference between isoflurane and sevoflurane groups regarding hemodynamic and temperature variables (P > 0.05).
Sevoflurane significantly increased DNA damage compared to unexposed animals (P = 0.02).
In addition, Wistar rats anesthetized with isoflurane showed higher antioxidative status (MTT) than control group (P = 0.019).
There were no significant differences in DNA damage or antioxidant status between isoflurane and sevoflurane groups (P > 0.05).
In conclusion, our findings suggest that, in contrast to sevoflurane exposure, isoflurane increases systemic antioxidative status, protecting cells from DNA damage in rats.
American Psychological Association (APA)
Rocha, Thalita L. A.& Dias-Junior, Carlos A.& Possomato-Vieira, Jose S.& Gonçalves-Rizzi, Victor H.& Nogueira, Flávia R.& de Souza, Kátina M.…[et al.]. 2015. Sevoflurane Induces DNA Damage Whereas Isoflurane Leads to Higher Antioxidative Status in Anesthetized Rats. BioMed Research International،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1054812
Modern Language Association (MLA)
Rocha, Thalita L. A.…[et al.]. Sevoflurane Induces DNA Damage Whereas Isoflurane Leads to Higher Antioxidative Status in Anesthetized Rats. BioMed Research International No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1054812
American Medical Association (AMA)
Rocha, Thalita L. A.& Dias-Junior, Carlos A.& Possomato-Vieira, Jose S.& Gonçalves-Rizzi, Victor H.& Nogueira, Flávia R.& de Souza, Kátina M.…[et al.]. Sevoflurane Induces DNA Damage Whereas Isoflurane Leads to Higher Antioxidative Status in Anesthetized Rats. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1054812
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054812