Epitope Fingerprinting for Recognition of the Polyclonal Serum Autoantibodies of Alzheimer’s Disease
Joint Authors
Ueira-Vieira, Carlos
de Oliveira-Júnior, Luiz Carlos
Araújo Santos, Fabiana de Almeida
Goulart, Luiz Ricardo
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Autoantibodies (aAb) associated with Alzheimer’s disease (AD) have not been sufficiently characterized and their exact involvement is undefined.
The use of information technology and computerized analysis with phage display technology was used, in the present research, to map the epitope of putative self-antigens in AD patients.
A 12-mer random peptide library, displayed on M13 phages, was screened using IgG from AD patients with two repetitions.
Seventy-one peptides were isolated; however, only 10 were positive using the Elisa assay technique (Elisa Index > 1).
The results showed that the epitope regions of the immunoreactive peptides, identified by phage display analysis, were on the exposed surfaces of the proteins.
The putative antigens MAST1, Enah, MAO-A, X11/MINT1, HGF, SNX14, ARHGAP 11A, APC, and CENTG3, which have been associated with AD or have functions in neural tissue, may indicate possible therapeutic targets.
American Psychological Association (APA)
de Oliveira-Júnior, Luiz Carlos& Araújo Santos, Fabiana de Almeida& Goulart, Luiz Ricardo& Ueira-Vieira, Carlos. 2015. Epitope Fingerprinting for Recognition of the Polyclonal Serum Autoantibodies of Alzheimer’s Disease. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054821
Modern Language Association (MLA)
de Oliveira-Júnior, Luiz Carlos…[et al.]. Epitope Fingerprinting for Recognition of the Polyclonal Serum Autoantibodies of Alzheimer’s Disease. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1054821
American Medical Association (AMA)
de Oliveira-Júnior, Luiz Carlos& Araújo Santos, Fabiana de Almeida& Goulart, Luiz Ricardo& Ueira-Vieira, Carlos. Epitope Fingerprinting for Recognition of the Polyclonal Serum Autoantibodies of Alzheimer’s Disease. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054821
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054821