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Yeast Actin-Related Protein ARP6 Negatively Regulates Agrobacterium-Mediated Transformation of Yeast Cell
Joint Authors
Luo, Yumei
Zhu, Detu
Chen, Zikai
Tu, Haitao
Pan, Shen Quan
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-06
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The yeasts, including Saccharomyces cerevisiae and Pichia pastoris, are single-cell eukaryotic organisms that can serve as models for human genetic diseases and hosts for large scale production of recombinant proteins in current biopharmaceutical industry.
Thus, efficient genetic engineering tools for yeasts are of great research and economic values.
Agrobacterium tumefaciens-mediated transformation (AMT) can transfer T-DNA into yeast cells as a method for genetic engineering.
However, how the T-DNA is transferred into the yeast cells is not well established yet.
Here our genetic screening of yeast knockout mutants identified a yeast actin-related protein ARP6 as a negative regulator of AMT.
ARP6 is a critical member of the SWR1 chromatin remodeling complex (SWR-C); knocking out some other components of the complex also increased the transformation efficiency, suggesting that ARP6 might regulate AMT via SWR-C.
Moreover, knockout of ARP6 led to disruption of microtubule integrity, higher uptake and degradation of virulence proteins, and increased DNA stability inside the cells, all of which resulted in enhanced transformation efficiency.
Our findings have identified molecular and cellular mechanisms regulating AMT and a potential target for enhancing the transformation efficiency in yeast cells.
American Psychological Association (APA)
Luo, Yumei& Chen, Zikai& Zhu, Detu& Tu, Haitao& Pan, Shen Quan. 2015. Yeast Actin-Related Protein ARP6 Negatively Regulates Agrobacterium-Mediated Transformation of Yeast Cell. BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1054844
Modern Language Association (MLA)
Luo, Yumei…[et al.]. Yeast Actin-Related Protein ARP6 Negatively Regulates Agrobacterium-Mediated Transformation of Yeast Cell. BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1054844
American Medical Association (AMA)
Luo, Yumei& Chen, Zikai& Zhu, Detu& Tu, Haitao& Pan, Shen Quan. Yeast Actin-Related Protein ARP6 Negatively Regulates Agrobacterium-Mediated Transformation of Yeast Cell. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1054844
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054844