Identification of the Alternative Splicing of the UL49 Locus of Human Cytomegalovirus
Joint Authors
Yang, Guang
Li, Wei
Liao, Wenzhen
Zhang, Xin
Zou, Yi
Li, Yueqin
Jing, Chunxia
Zhou, Tianhong
Dai, Jianfeng
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-19
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The UL49 ORF of human cytomegalovirus (HCMV) is essential for viral replication; conserved among all herpes viruses; however, the function is unclear.
Once the UL49 ORF was precisely deleted from the start to stop codon, the mutant did not yield infectious progeny.
In this study, we find out many alternatively processed ESTs in UL49 locus in HCMV-infected cells, in which there are two novel transcription termination sites in UL49 locus.
Most of these ESTs are rare transcripts that contain directed repeat sequences in the intron splicing regions.
There is a typical GU-AG intron splicing site in UL49Y transcripts.
The 1847 bp UL49Y cDNA spans an ORF from 335 to 1618 and encodes a putative protein of 427 amino acids with a predicted molecular mass of 47.1 kDa.
All the new EST sequences and UL49Y cDNA sequence have been deposited in the GenBank database (GenBank Accession nos.
GW314860-GW314900 and GU376796).
This study provides us with very important clues for revealing the importance of the UL49 locus alternative splicing.
American Psychological Association (APA)
Yang, Guang& Li, Wei& Liao, Wenzhen& Zhang, Xin& Zou, Yi& Dai, Jianfeng…[et al.]. 2015. Identification of the Alternative Splicing of the UL49 Locus of Human Cytomegalovirus. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1054859
Modern Language Association (MLA)
Yang, Guang…[et al.]. Identification of the Alternative Splicing of the UL49 Locus of Human Cytomegalovirus. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1054859
American Medical Association (AMA)
Yang, Guang& Li, Wei& Liao, Wenzhen& Zhang, Xin& Zou, Yi& Dai, Jianfeng…[et al.]. Identification of the Alternative Splicing of the UL49 Locus of Human Cytomegalovirus. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1054859
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054859