Muscle IGF-1-Induced Skeletal Muscle Hypertrophy Evokes Higher Insulin Sensitivity and Carbohydrate Use as Preferential Energy Substrate
Joint Authors
Musarò, Antonio
Christoffolete, Marcelo Augusto
Silva, William Jose
Ramos, Gracielle Vieira
Bento, Mirella Ribeiro
Costa, Monique Oliveira
Ribeiro, Miriam Oliveira
Okamoto, Maristela Mitiko
Lohmann, Tania Helena
Machado, Ubiratan Fabres
Moriscot, Anselmo Sigari
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-04
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
We characterized the metabolic profile of transgenic mice exhibiting enhanced muscle mass driven by increased mIGF-1 expression (MLC/mIGF-1).
As expected, 6-month-old MLC/mIGF-1 mice were heavier than age-matched wild type (WT) mice (37.4 ± 0.3 versus 31.8 ± 0.6 g, resp.).
MLC/mIGF-1 mice had higher respiratory quotient when compared to WT (0.9 ± 0.03 versus 0.74 ± 0.02, resp.) suggesting a preference for carbohydrate as the major fuel source.
MLC/mIGF-1 mice had a higher rate of glucose disposal when compared to WT (3.25 ± 0.14 versus 2.39 ± 0.03%/min, resp.).
The higher disposal rate correlated to ∼2-fold higher GLUT4 content in the extensor digitorum longus (EDL) muscle.
Analysis of mRNA content for the glycolysis-related gene PFK-1 showed ∼3-fold upregulation in MLC/mIGF-1 animals.
We also found a 50% downregulation of PGC1α mRNA levels in MLC/mIGF-1 mouse EDL muscle, suggesting less abundant mitochondria in this tissue.
We found no difference in the expression of PPARα and PPARβ/δ, suggesting no modulation of key elements in oxidative metabolism.
These data together suggest a shift in metabolism towards higher carbohydrate utilization, and that could explain the increased insulin sensitivity of hypertrophied skeletal muscle in MLC/mIGF-1 mice.
American Psychological Association (APA)
Christoffolete, Marcelo Augusto& Silva, William Jose& Ramos, Gracielle Vieira& Bento, Mirella Ribeiro& Costa, Monique Oliveira& Ribeiro, Miriam Oliveira…[et al.]. 2015. Muscle IGF-1-Induced Skeletal Muscle Hypertrophy Evokes Higher Insulin Sensitivity and Carbohydrate Use as Preferential Energy Substrate. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054874
Modern Language Association (MLA)
Christoffolete, Marcelo Augusto…[et al.]. Muscle IGF-1-Induced Skeletal Muscle Hypertrophy Evokes Higher Insulin Sensitivity and Carbohydrate Use as Preferential Energy Substrate. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1054874
American Medical Association (AMA)
Christoffolete, Marcelo Augusto& Silva, William Jose& Ramos, Gracielle Vieira& Bento, Mirella Ribeiro& Costa, Monique Oliveira& Ribeiro, Miriam Oliveira…[et al.]. Muscle IGF-1-Induced Skeletal Muscle Hypertrophy Evokes Higher Insulin Sensitivity and Carbohydrate Use as Preferential Energy Substrate. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054874
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054874